Product Details

• Product Name: Recombinant Human VEGF (121aa)
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z102115
• Unit Size: 10 µg
• Species: Human (Homo sapiens)
• Gene Symbol: VEGFA
• Gene ID: 7422
• Accession Number: P15693
• Expression System: E. coli
• Molecular Weight: ~14.3 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from 0.2 μm filtered solution in Tris and NaCl, pH 8.0
• Function: Induces proliferation of human umbilical vein endothelial cells (HUVEC) with ED₅₀ ~10 ng/mL
• Storage:
  • Lyophilized: –70°C (stable for ≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human VEGF121 is a biologically active, non-heparin-binding isoform of Vascular Endothelial Growth Factor A. This homodimeric glycoprotein plays a central role in angiogenesis and endothelial cell function by stimulating mitogenesis via VEGF receptors. VEGF121, lacking the heparin-binding domain of larger isoforms, is fully soluble and particularly useful for controlled in vitro vascular studies. Expressed in E. coli and highly purified, this recombinant cytokine supports a wide variety of vascular biology, tissue regeneration, and cancer-related applications.

Key Features and Benefits

• Validated Bioactivity: Promotes dose-dependent HUVEC proliferation (ED₅₀ ≈ 10 ng/mL).
• Soluble Isoform: VEGF121 does not bind heparin, offering consistent activity in solution-phase assays.
• High Purity, Low Endotoxin: >95% purity by SDS-PAGE; endotoxin level <1 EU/µg.
• Stable Lyophilized Format: Long shelf-life and flexible storage options.
• Reliable Expression: Recombinant production in E. coli enables scalable research-grade consistency.

Reconstitution Instructions

1. Briefly centrifuge vial to collect contents.
2. Reconstitute with sterile distilled water to ≥ 0.1 mg/mL.
3. Gently swirl to dissolve—do not vortex.
4. For downstream use, further dilution in compatible buffer or media is recommended.
5. Filter sterilization may be required based on application.

Quality Control (QC)

• Purity validated by SDS-PAGE
• Endotoxin tested via LAL method
• Activity confirmed through HUVEC proliferation assay

Caution

This product is intended strictly for research purposes. It is not suitable for diagnostic, therapeutic, or clinical use in humans or animals.

Disclaimer

1. This product is provided for research use only. Not for human or veterinary use. Not intended for clinical, diagnostic, or therapeutic applications.
2. The manufacturer assumes no liability for any unauthorized use. It is the user’s responsibility to comply with applicable regulations and safety practices.