Product Details

• Product Name: Recombinant Human MIF (E. coli)
• Product Type: Cytokines & Immune Modulators
• Catalog Number: Z100915
• Unit Size: 25 µg
• Species: Human (Homo sapiens)
• Gene Symbol: MIF
• Gene ID: 4282
• Accession Number: P14174
• Aliases: GIF, GLIF, MMIF
• Expression System: E. coli
• Molecular Weight: ~12.3 kDa (monomer)
• Form: Lyophilized powder; white to off-white appearance
• Purity: >95% (SDS-PAGE)
• Endotoxin Level: <1.0 EU/µg (LAL method)
• Formulation: Lyophilized from a 0.2 μm filtered PBS solution, pH 7.4
• Function: Activity determined by ability to bind recombinant human CD74 in a functional ELISA
• Reconstitution: Briefly spin; reconstitute with sterile distilled water to ≥ 0.1 mg/mL; dilute with desired buffer
• Storage:
  • Lyophilized: −70 °C, stable for ≥ 12 months from receipt
  • After reconstitution: 2–8 °C (≤ 1 month) or −20 °C (≤ 6 months with a carrier protein); avoid repeated freeze/thaw cycles
• Usage: For research use only; not for diagnostic or therapeutic applications
• Shipping: Dry ice

Overview

Macrophage Migration Inhibitory Factor (MIF) is a pleiotropic cytokine rapidly released at inflammatory sites to shape innate and adaptive immune responses, including regulation of macrophage activation and counteraction of glucocorticoid-mediated immunosuppression. In vitro, MIF displays phenylpyruvate and dopachrome tautomerase activities, though the in-vivo relevance remains uncertain. Recombinant Human MIF expressed in E. coli provides a consistent, bioactive reagent for studies of immune regulation, inflammatory signaling, and disease pathogenesis.

Key Features and Benefits

• Functionally Verified: Demonstrates specific binding to recombinant human CD74 in a functional ELISA, confirming activity.
• High Quality: >95% purity by SDS-PAGE with endotoxin <1.0 EU/µg (LAL), supporting sensitive cell-based assays.
• Stable, Ready-to-Use Format: Supplied as a lyophilized powder for flexible reconstitution and long-term storage.
• Broad Utility: Suitable for mechanistic studies of cytokine signaling, macrophage biology, and inflammation-driven disease models.
• Recombinant Consistency: Bacterial expression enables batch-to-batch reproducibility.

Reconstitution Instructions

To prepare for use, follow these procedures carefully:

• Centrifuge the Vial: Briefly spin to collect material at the bottom.
• Reconstitute: Add sterile distilled water to achieve a final concentration of ≥ 0.1 mg/mL; swirl gently to dissolve completely. Do not vortex.
• Dilution and Application: Dilute the reconstituted solution into the desired buffer or culture medium for immediate use.
• Post-Reconstitution Storage: 2–8 °C for up to 1 month, or −20 °C for up to 6 months with a suitable carrier protein; avoid repeated freeze/thaw cycles.

Quality Control (QC)

• Purity confirmed by SDS-PAGE (>95%).
• Endotoxin level measured by LAL assay (<1.0 EU/µg).
• Functional activity verified by CD74 binding in a functional ELISA.

Caution

This product is intended strictly for research purposes. It is not approved for human or veterinary use and must not be used for diagnostic, therapeutic, or clinical procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not intended for diagnostic or therapeutic applications.
4. Users are solely responsible for appropriate handling and regulatory compliance.