Product Details

• Product Name: Recombinant Human IL1B
• Product Type: Cytokines
• Catalog Number: Z100395
• Unit Size: 10 µg
• Species: Human (Homo sapiens)
• Gene Symbol: IL1B
• Gene ID: 3553
• Accession Number: P01584
• Expression System: E. coli
• Molecular Weight: ~17.5 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from 0.2 μm filtered solution in Tris, NaCl, EDTA and DTT (pH 8.0)
• Function: Demonstrates dose-dependent inhibition of prostate carcinoma cells with ED₅₀ < 1 ng/mL
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Interleukin-1 Beta (IL1B) is a potent immunomodulatory cytokine involved in the regulation of inflammatory responses. It is primarily secreted by monocytes and macrophages and plays a vital role in host defense by promoting fever, activating endothelial cells, and stimulating the production of acute-phase proteins.

IL1B functions as a monomer and must be enzymatically cleaved from its precursor form to become biologically active. It shares a receptor with IL1A, and both isoforms elicit similar signaling effects. IL1B has been shown to stimulate the release of prostaglandins and collagenases from synovial cells, induce thymocyte proliferation, B-cell maturation, and fibroblast growth factor activity, making it essential for studying inflammatory and autoimmune pathways.

Key Features and Benefits

• Proven Biological Activity: Demonstrates ED₅₀ < 1 ng/mL based on inhibition of prostate carcinoma cell proliferation.
• High Purity, Low Endotoxin: Purity > 95% verified by SDS-PAGE; endotoxin content < 1.0 EU/µg, suitable for sensitive cell assays.
• Inflammation Research Tool: Facilitates exploration of cytokine-mediated responses in innate immunity and autoimmunity.
• Receptor Binding Fidelity: Binds IL1 receptor to mimic native IL1B signaling without loss of activity.
• Stable Format: Supplied as a lyophilized powder for extended shelf life and convenient reconstitution.

Reconstitution Instructions

To prepare for use, follow these procedures carefully:

• Centrifuge the Vial: Briefly spin the vial to collect the contents.
• Reconstitute: Add sterile distilled water to achieve a concentration ≥ 0.1 mg/mL.
• Dissolution: Swirl gently to dissolve the protein completely. Do not vortex.
• Dilution and Application: Further dilute using the desired buffer or medium for experimental use.
• Storage: After reconstitution, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months (with carrier protein). Avoid freeze/thaw cycles.

Quality Control (QC)

• SDS-PAGE confirmed purity > 95%
• Endotoxin level verified by LAL assay (< 1.0 EU/µg)
• Biological activity validated using carcinoma cell inhibition assay

Caution

This product is intended for research purposes only. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not intended for diagnostic or therapeutic applications.
4. Users are solely responsible for safety and regulatory compliance.