Product Details

• Product Name: Recombinant Human IL15 (E. coli)
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z100455
• Unit Size: 10 µg
• Species: Human (Homo sapiens)
• Gene Symbol: IL15
• Gene ID: 3600
• Accession Number: P40933
• Expression System: E. coli
• Molecular Weight: ~13 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from PBS, pH 7.5 (0.2 μm filtered)
• Function: Potent inducer of T lymphocyte proliferation (ED₅₀ <0.1 ng/mL)
• Storage:
  • Lyophilized: stable for ≥12 months at –70°C
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Interleukin-15 (IL-15) is a pleiotropic cytokine with critical roles in both adaptive and innate immunity. It is produced by astrocytes, microglial cells, and other immune-related cells under inflammatory conditions. IL-15 drives the proliferation and survival of CD8⁺ memory T cells, enhances natural killer (NK) cell development and cytotoxicity, and can substitute for bone marrow microenvironmental signals in vitro. Unlike IL-2, IL-15 promotes mast cell proliferation via distinct receptor signaling mechanisms involving JAK2 and STAT5. Its unique biology underscores its importance in immune regulation and translational research in immunology, oncology, and infectious disease.

Key Features and Benefits

• Potent Bioactivity: ED₅₀ <0.1 ng/mL for T lymphocyte proliferation.
• High Purity and Low Endotoxin: Optimized for sensitive immunological assays.
• Broad Research Relevance: Suitable for studies of T cell biology, NK cell development, and tumor immunology.
• Stable Lyophilized Form: Maintains activity and long-term storage stability.
• Tag-Free Preparation: Preserves native structural and receptor-binding characteristics.

Reconstitution Instructions

To ensure optimal activity and performance:

1. Centrifuge the vial: Briefly spin to collect all lyophilized material at the bottom.
2. Reconstitute: Add sterile distilled water to a final concentration of ≥0.1 mg/mL.
3. Mixing: Gently swirl or tap the vial until dissolved; do not vortex.
4. Dilution: Prepare working aliquots in suitable buffer or culture medium.
5. Storage: After reconstitution, store at 2–8°C for ≤1 month, or at –20°C for ≤6 months with carrier protein. Avoid repeated freeze/thaw cycles.

Quality Control (QC)

• Purity confirmed by SDS-PAGE
• Endotoxin quantified by LAL assay
• Bioactivity validated in T lymphocyte proliferation assay

Caution

This product is intended for laboratory research use only. It is not approved for diagnostic, therapeutic, or clinical applications in humans or animals.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not intended for diagnostic or therapeutic purposes.
4. No warranties are expressed or implied beyond intended use.
5. Users are responsible for compliance with all applicable regulations.