Product Details

• Product Name: Recombinant Human IL-2
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z100485
• Unit Size: 50 µg
• Species: Human (Homo sapiens)
• Gene Symbol: IL2
• Gene ID: 3558
• Accession Number: P60568
• Source: E. coli
• Molecular Weight: ~ 15.5 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from a 0.2 µm-filtered solution in acetic acid
• Function: Stimulates CTLL-2 proliferation at ED₅₀ < 0.5 ng/mL
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Interleukin-2 (IL-2) is a critical cytokine primarily involved in the activation and expansion of T cells, NK cells, and B cells. It plays an essential role in immune tolerance and inflammation, making it a valuable tool in immunological studies and preclinical models. Produced in E. coli, this cytokine is purified to high standards for optimal reproducibility in cell-based experiments.

Key Features and Benefits

• High Potency: Demonstrates ED₅₀ < 0.5 ng/mL in CTLL-2 proliferation assay.
• Endotoxin-Safe: < 0.1 EU/µg for sensitive cell cultures.
• Research-Grade Purity: > 95% by SDS-PAGE.
• Reliable Activity: Consistent batch-to-batch bioactivity validation.
• Flexible Handling: Lyophilized format for stable storage and reconstitution.

Reconstitution Instructions

To ensure maximum protein activity and stability, follow these steps:

1. Centrifuge the Vial: Briefly spin down to collect lyophilized content.
2. Add sterile solvent: Reconstitute using 100 mM sterile acetic acid to achieve a final concentration of 0.1–1.0 mg/mL.
3. Mix gently: Swirl or tap to dissolve. Avoid vortexing.
4. Dilute as needed: Further dilute in carrier protein-containing buffer for applications.
5. Storage: Reconstituted solution can be stored at 2–8°C (1 month) or –20°C (6 months).
   • Avoid repeated freeze-thaw cycles.

Quality Control (QC)

• Purity confirmed by SDS-PAGE
• Endotoxin level verified using LAL assay
• Bioactivity validated via CTLL-2 proliferation bioassay

 Caution

This product is intended for research purposes only. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not for diagnostic or therapeutic purposes.
4. The supplier assumes no liability for misuse of the product.
5. Users are solely responsible for compliance with all applicable regulations.