Product Details

• Product Name: Recombinant Human IGF1
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z100385
• Unit Size: 100 µg
• Species: Human (Homo sapiens)
• Gene Symbol: IGF1
• Gene ID: 3479
• Accession Number: P01343
• Source: E. coli
• Molecular Weight: ~7.7 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White lyophilized powder
• Formulation: Lyophilized from 0.2 µm filtered solution in sodium phosphate (pH 6.5) and 150 mM NaCl
• Activity: ED₅₀ ≤ 20 ng/mL (MCF-7 proliferation assay)
• Storage:
  • Lyophilized: –70°C (stable ≥ 12 months)
  • After Reconstitution: 2–8°C (≤ 1 month), –20°C (≤ 6 months with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Insulin-like Growth Factor 1 (IGF1) is a key regulator of cellular growth, differentiation, and survival. It mimics endogenous IGF1 in promoting anabolic responses, particularly in muscle, cartilage, and bone tissues. Produced in E. coli, this recombinant form represents the mature IGF1 protein, free of pro-peptide regions and structurally stabilized with native disulfide bonds. Its cross-species activity makes it suitable for a wide range of in vitro biomedical applications, including growth hormone response studies and tissue modeling.

Key Features and Benefits

• High Activity: Potent mitogenic effects verified by ED₅₀ ≤ 20 ng/mL in MCF-7 cells.
• Purity and Safety: >95% purity with low endotoxin, suitable for sensitive cell-based assays.
• Cross-Species Utility: Effective across human, mouse, and rat systems.
• Reliable Performance: Batch consistency for reproducible experimental outcomes.
• Flexible Handling: Long shelf life in lyophilized form with reconstitution stability.

Reconstitution Instructions

1. Briefly centrifuge the vial to collect contents at the bottom.
2. Add sterile distilled water to achieve ≥0.1 mg/mL concentration.
3. Gently swirl or tap to dissolve (do not vortex).
4. Use freshly reconstituted solution or store according to recommended conditions.

Quality Control (QC)

• Verified purity by SDS-PAGE
• Endotoxin tested by LAL method
• Functional activity confirmed via MCF-7 proliferation assay

Caution

This product is intended for research purposes only. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not intended for diagnostic or therapeutic applications.
4. Users are responsible for compliance with local regulations and safe laboratory practices.