Product Details

• Product Name: Recombinant Human IFNG
• Product Type: Cytokines & Immunomodulators
• Catalog Number: Z101175
• Unit Size: 200 µg
• Species: Human (Homo sapiens)
• Gene Symbol: IFNG
• Gene ID: 3458
• Accession Number: P01579
• Aliases: IFG, IFI, IMD69
• Source: CHO cells
• Molecular Weight: ~17 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White lyophilized powder
• Formulation: Lyophilized from 0.2 µm-filtered PBS (pH 7.2) at 1.0 mg/mL
• Function: Demonstrated antiviral activity with ED₅₀ ≤ 1.0 ng/mL in mouse L929 viral resistance assay; specific activity ≥ 1.0 × 10⁶ units/mg
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution: 2–8°C (≤ 1 month) or –20°C (≤ 6 months, with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Interferon-Gamma (IFNG) is a critical immune mediator predominantly secreted by activated T lymphocytes and natural killer cells. It orchestrates innate and adaptive immunity by enhancing antigen presentation, upregulating MHC expression, and activating macrophages. IFN-γ also possesses potent antiviral, antiparasitic, and antiproliferative properties. Functionally, it works synergistically with IL-1, IL-2, and TNF family cytokines to amplify immune responses. This CHO cell-derived recombinant form retains proper folding and post-translational modifications, ensuring high bioactivity for in vitro immunology and infectious disease research.

Key Features and Benefits

• Biologically Active: Exhibits ED₅₀ ≤ 1.0 ng/mL based on mouse L929 viral resistance assay.
• Low Endotoxin Level: < 1.0 EU/µg, suitable for sensitive immune cell applications.
• High Purity: > 95% verified by SDS-PAGE ensures reproducible results.
• Validated Immune Modulation: Proven to enhance antigen presentation and macrophage activation in vitro.
• Stable Lyophilized Form: Supports long-term storage and consistent experimental performance.

Reconstitution Instructions

To ensure optimal bioactivity and stability:

1. Centrifuge the Vial: Briefly spin to collect the powder at the bottom.
2. Reconstitute: Add sterile distilled water to a final concentration ≥ 0.1 mg/mL.
3. Mix Gently: Swirl to dissolve; avoid vigorous shaking or vortexing.
4. Dilute for Use: Further dilute in the desired buffer containing carrier protein.
5. Storage: Store reconstituted protein at 2–8°C (≤ 1 month) or –20°C (≤ 6 months).

Quality Control (QC)

• Purity: Verified by SDS-PAGE
• Endotoxin: Measured using Limulus Amebocyte Lysate (LAL) method
• Bioactivity: Confirmed in viral resistance assay with mouse L929 cells

Caution

This product is intended for research use only. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not for diagnostic or therapeutic purposes.
4. The supplier assumes no liability for the misuse of this product.
5. Users are responsible for compliance with institutional and regulatory guidelines.