Product Details

• Product Name: Recombinant Human GM-CSF (CSF2)
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z100275
• Unit Size: 20 µg
• Species: Human (Homo sapiens)
• Gene Symbol: CSF2
• Gene ID: 1437
• Accession Number: P04141
• Expression System: E. coli
• Molecular Weight: ~15 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from 0.2 μm filtered solution containing Tris and NaCl
• Function: Promotes proliferation of TF-1 cells (ED₅₀ ≤ 0.5 ng/mL)
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) is a biologically active cytokine that plays an essential role in the development and activation of granulocyte and macrophage progenitor cells. Produced in E. coli, this non-glycosylated form mimics the natural protein’s in vitro function, supporting immune cell expansion and functional assays in hematopoietic studies. GM-CSF is known to modulate the immune response and is often employed in research focused on hematologic diseases, immunotherapy, and stem cell biology.

Key Features and Benefits

• Validated Bioactivity: Induces TF-1 cell proliferation at low nanogram concentrations (ED₅₀ ≤ 0.5 ng/mL).
• Efficient Expression: Bacterial expression system ensures scalable, cost-effective production.
• High Purity & Low Endotoxin: >95% purity and endotoxin levels <1 EU/µg, ideal for sensitive cell culture work.
• Functional Versatility: Suitable for hematopoiesis, myeloid cell development, and immunological assays.
• Stable Form: Lyophilized format for long-term storage and flexible experimental planning.

Reconstitution Instructions

To ensure optimal performance:

1. Brief Spin: Centrifuge vial to collect contents at the bottom.
2. Reconstitute: Add sterile distilled water to achieve a final concentration of ≥0.1 mg/mL.
3. Mix Gently: Swirl until completely dissolved; do not vortex.
4. Application: Dilute in appropriate assay buffer or culture medium as required.

Quality Control (QC)

• Purity assessed via SDS-PAGE
• Endotoxin levels measured by LAL assay
• Functional activity validated in TF-1 cell proliferation assay

Caution

This product is intended strictly for research use only. It is not to be used in diagnostic or therapeutic applications involving humans or animals.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not intended for diagnostic, therapeutic, or clinical applications.
4. Users are solely responsible for ensuring proper regulatory compliance and safety in their research practices.