Product Details

• Product Name: Recombinant Human FGF2
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z101455
• Unit Size: 50 µg
• Species: Human (Homo sapiens)
• Gene Symbol: FGF2
• Gene ID: 2247
• Accession Number: P09038
• Expression System: E. coli
• Molecular Weight: ~17 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from 0.2 µm filtered PBS solution
• Function: Stimulates NIH/3T3 cell proliferation with ED₅₀ typically in the range of 0.05 – 0.5 ng/mL
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months with 0.1% BSA)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Fibroblast Growth Factor 2 (FGF2), also known as basic FGF (bFGF), is a multifunctional growth factor involved in cell proliferation, differentiation, and angiogenesis. Acting through FGFR family receptors, FGF2 is essential in developmental biology, tissue repair, and cancer research. Produced in E. coli, this recombinant version retains potent mitogenic activity suitable for a wide range of in vitro applications.

Key Features and Benefits

• Confirmed Activity: Promotes NIH/3T3 cell proliferation (ED₅₀ = 0.05–0.5 ng/mL).
• Efficient Expression System: Bacterial production enables cost-effective and scalable research applications.
• High Purity, Low Endotoxin: SDS-PAGE confirmed purity > 95%; LAL-tested endotoxin < 1 EU/µg.
• Versatile Use: Supports studies in stem cell biology, angiogenesis, tissue engineering, and neuroscience.
• Stable Format: Lyophilized for long-term storage and convenient reconstitution.

Reconstitution Instructions

To prepare for use, follow these procedures carefully:

1. Centrifuge the Vial:
   • Spin briefly to ensure powder is collected at the bottom.
2. Reconstitute:
   • Add sterile distilled water to achieve a final concentration of ≥ 0.1 mg/mL.
   • Swirl gently to dissolve completely. Do not vortex.
   • Note: Filter sterilization is required post-reconstitution.
3. Dilution and Application:
   • Dilute reconstituted solution with desired buffer or media as needed.
4. Storage:
   • Store reconstituted solution at 2–8°C for up to 1 month, or –20°C for up to 6 months with 0.1% BSA.
   • Avoid repeated freeze/thaw cycles.

Quality Control (QC)

• Purity confirmed by SDS-PAGE
• Endotoxin level determined by LAL assay
• Bioactivity validated in NIH/3T3 cell proliferation assay

Caution

This product is intended for research purposes only. It is not to be used in humans or animals for therapeutic or diagnostic procedure.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not intended for diagnostic or therapeutic applications.
4. No responsibility is assumed for any use other than research.
5. Users are solely responsible for safety and regulatory compliance.

References

1. Shen, Jun, et al. “Defining early hematopoietic‐fated primitive streak specification of human pluripotent stem cells by the orchestrated balance of Wnt, activin, and BMP signaling.” Journal of cellular physiology9 (2019): 16136-16147.