Recombinant Human CCL2

Product Details

Product Name: Recombinant Human CCL2 (E. coli)
Product Type: Chemokines & Cytokines
Catalog Number: Z100625
Unit Size: 20 µg
Species: Human (Homo sapiens)
Gene Symbol: CCL2
Gene ID: 6347
Accession Number: P13500
Source: E. coli
Molecular Weight: ~9 kDa
Form: Lyophilized powder
Purity: >95% (SDS-PAGE)
Endotoxin Level: <1.0 EU/µg (LAL method)
Appearance: White to off-white lyophilized powder
Formulation: Lyophilized from PBS, pH 7.4
Function: Induces dose-dependent proliferation of PC3 cells (ED₅₀ < 0.2 ng/mL)
Storage:
- After reconstitution:
  - 2–8°C (≤1 month)
  - –20°C (≤6 months) with carrier protein
- Avoid repeated freeze–thaw cycles
Usage: For research use only; not for diagnostic or therapeutic applications

Overview

CCL2, also known as monocyte chemoattractant protein-1 (MCP-1), is a β-chemokine secreted by a broad range of cell types, including monocytes, endothelial cells, and fibroblasts. It functions primarily to direct the migration of monocytes, memory T cells, and dendritic cells to sites of inflammation. In addition to chemotaxis, CCL2 modulates tumor–immune interactions, influences cytokine expression, and acts as an activator for certain innate immune cells.

Key Features and Benefits

Potent Chemotactic Activity: Validated stimulation of PC3 cell proliferation (ED₅₀ < 0.2 ng/mL).
High Purity, Low Endotoxin: Minimizes background effects in sensitive assays.
Versatile Research Utility: Suitable for inflammation, tumor biology, and immune regulation studies.
Stable Lyophilized Form: Optimized for long-term storage and reproducible performance.
Tag-free Preparation: Preserves natural protein structure and receptor interactions.

Quality Control (QC)

Purity confirmed via SDS-PAGE
Endotoxin level determined by LAL assay
Bioactivity validated through cell-based proliferation assay

Caution

This product is intended for laboratory research purposes only. Not for use in human or animal diagnostic, therapeutic, or clinical procedures.

Disclaimer

For research use only.
Not for human or veterinary applications.
Not for therapeutic or diagnostic use.
No warranties are expressed or implied beyond the intended purpose.
Users are responsible for compliance with all applicable regulations.