Product Details

• Product Name: Recombinant Human Beta-NGF (E. coli)
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z102165
• Unit Size: 20 µg
• Species: Human (Homo sapiens)
• Gene Symbol: NGF
• Gene ID: 4803
• Accession Number: P01138
• Source: E. coli expression system
• Molecular Weight: ~14 kDa
• Form: Lyophilized powder
• Purity: >95% (SDS-PAGE)
• Endotoxin Level: <1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from 0.2 μm filtered solution containing 0.1% TFA
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months, with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Beta-Nerve Growth Factor (β-NGF) is a critical neurotrophin that regulates neuronal growth, survival, and differentiation. It also plays a role in the immune system, acting as a growth and differentiation factor for B lymphocytes. β-NGF is a member of the cysteine-knot family, forming stable disulfide-linked homodimers.

Human β-NGF shares ~90% amino acid sequence homology with rodent NGF, allowing for cross-species applications in neurobiology research. In vitro, β-NGF has been shown to promote the survival and differentiation of neuronal cell populations. This recombinant protein, produced in E. coli, is designed for high activity, purity, and consistency, making it suitable for diverse studies in neuroscience and immunology.

Key Features and Benefits

• High Biological Activity: Supports neuronal differentiation and maintenance in vitro.
• Immunomodulatory Properties: Enhances B cell survival and function.
• High Purity & Low Endotoxin: >95% purity, <1.0 EU/µg endotoxin, suitable for sensitive assays.
• Cross-Species Utility: High sequence homology with mouse and rat NGF.
• Stable Lyophilized Form: Convenient long-term storage and reliable reconstitution.

Reconstitution Instructions

To maintain optimal quality and bioactivity, please follow these guidelines:

1. Centrifuge the Vial: Briefly spin to collect all lyophilized material at the bottom.
2. Reconstitute: Add sterile distilled water to a final concentration of ≥0.1 mg/mL.
3. Mixing: Gently swirl or tap the vial until fully dissolved. Do not vortex.
4. Dilution: Prepare working solutions by diluting in appropriate buffer or culture medium.
5. Storage: Store reconstituted protein at 2–8°C for ≤1 month, or at –20°C for ≤6 months in aliquots. Avoid repeated freeze/thaw cycles.

Quality Control (QC)

• Purity confirmed by SDS-PAGE
• Endotoxin quantified by LAL assay
• Bioactivity validated using established neuronal survival and differentiation assays

Caution

This product is intended for laboratory research use only. It is not approved for use in humans or animals for diagnostic, therapeutic, or clinical procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not intended for diagnostic or therapeutic purposes.
4. No warranties or guarantees are provided beyond intended use.
5. Users are responsible for all safety and regulatory compliance.