Product Details

• Product Name: Recombinant Human BDNF
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z100065
• Unit Size: 10 µg
• Species: Human (Homo sapiens)
• Gene Symbol: BDNF
• Gene ID: 627
• Accession Number: P23560
• Expression System: E. coli
• Molecular Weight: ~14 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from 0.2 μm filtered solution in citric acid
• Function: ED₅₀ determined by proliferation of rat C6 glioma cells; typical effective dose ~0.5 µg/mL
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family that selectively supports the survival and differentiation of sensory neurons and retinal ganglia. Highly expressed in the hippocampus and cortex, BDNF plays a crucial role in neurogenesis, synaptic modulation, and cognitive function. It exerts its biological effects primarily through TrkB receptors and has minimal interaction with the TrkA receptor that binds NGF. This recombinant BDNF, produced in E. coli, has demonstrated strong biological activity in neuronal cell assays and is suitable for a wide range of neuroscience research applications.

Key Features and Benefits

• Validated Activity: Stimulates rat C6 glioma cell proliferation with an ED₅₀ of ~0.5 µg/mL.
• Efficient Production: Bacterial expression system enables scalable and economical yields.
• High Purity: >95% by SDS-PAGE; endotoxin content below 1 EU/µg.
• Neuroscience-Relevant: Ideal for studies in neurodevelopment, neurodegeneration, and synaptic physiology.
• Stable Format: Lyophilized powder ensures long-term storage with preserved activity.

Reconstitution Instructions

To prepare the protein for use:

1. Spin the Vial: Briefly centrifuge to collect contents.
2. Reconstitute: Add sterile distilled water to a final concentration ≥ 0.1 mg/mL.
3. Dissolution: Gently mix until fully dissolved; avoid vortexing.
4. Dilution: Further dilute with appropriate buffer for downstream applications.
5. Storage: Store reconstituted solution at 2–8°C for up to 1 month, or at –20°C for up to 6 months with a carrier protein. Avoid freeze/thaw cycles.

Quality Control (QC)

• Purity verified by SDS-PAGE
• Endotoxin level determined using LAL assay
• Bioactivity validated via C6 glioma proliferation assay

Caution

This product is intended strictly for research purposes. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not intended for diagnostic or therapeutic applications.
4. The user assumes all responsibility for safety and compliance.