Product Details

• Product Name: Recombinant Human Adiponectin (Acrp30) (E. coli)
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z102595
• Unit Size: 25 µg
• Species: Human (Homo sapiens)
• Gene Symbol: ADIPOQ
• Gene ID: 9370
• Accession Number: Q15848
• Source: E. coli
• Molecular Weight: ~ 24.8 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from 0.2 µm filtered Tris buffer with NaCl, pH 8.0
• Function: ED₅₀ = 3.0–6.0 µg/mL in M1 cell cytotoxicity assay
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months, with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Adiponectin is a secreted protein predominantly produced by adipose tissue, recognized for enhancing insulin sensitivity and promoting fatty acid oxidation. As a member of the soluble defense collagen family, it influences adipocyte differentiation and exerts protective effects against cardiovascular disease by reducing vascular inflammation. Low circulating levels are associated with obesity and increased cardiovascular risk, making this recombinant form valuable for research in metabolic disorders, cardiovascular biology, and inflammatory regulation.

Key Features and Benefits

• Functionally Active: Demonstrates bioactivity in M1 cell cytotoxicity assays (ED₅₀ 3.0–6.0 µg/mL).
• Tag-Free Format: Preserves native protein structure and function.
• High Purity, Low Endotoxin: Suitable for sensitive in vitro applications.
• Recombinant in E. coli: Cost-effective production with reproducible quality.
• Broad Research Utility: Applicable to studies on obesity, insulin resistance, cardiovascular protection, and inflammation.

Reconstitution Instructions

1. Centrifuge the Vial: Briefly spin to collect all lyophilized material at the bottom.
2. Reconstitute: Add sterile deionized water to reach a final concentration of 0.1 mg/mL.
3. Mixing: Gently swirl or tap to fully dissolve; do not vortex.
4. Dilution and Application: After full dissolution, dilute in desired buffer or culture medium for experimental use.
5. Storage: Store reconstituted solution at 2–8°C for up to 1 month, or –20°C for up to 6 months (preferably in aliquots). Avoid freeze/thaw cycles.

Quality Control (QC)

• Purity verified by SDS-PAGE (> 95%).
• Endotoxin content measured via LAL assay (< 1.0 EU/µg).
• Biological activity confirmed in M1 cell cytotoxicity assay.

Caution

This product is intended for research use only. It is not to be used in human or veterinary medicine, nor for diagnostic or therapeutic purposes.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not for diagnostic or therapeutic applications.
4. No warranty is expressed or implied beyond the stated product specifications.
5. Users are solely responsible for compliance with local regulations and safe laboratory practices.