Immortalized Human T Cells

Product Details

Catalog Number: T0086
Unit Size: 1×10⁶ cells / 1.0 ml
Species: Human (Homo sapiens)
Tissue of Origin: Peripheral blood
Donor Information: Healthy human donor (anonymous)
Cell Type: Immortalized human T cells
Growth Properties: Suspension, forming rounded clumps
Immortalization Method: Lentiviral transduction with Lenti-hTERT (LV616) and Lenti-Rb siRNA (LV621)
Expression Markers: CD3 and HLA-27
Biosafety Level: BSL-2
Storage Condition: Below -130°C (liquid nitrogen vapor phase)
Shipping Condition: Shipped on dry ice
Format: Cryopreserved frozen cells

Overview

The Immortalized Human T Cells (T0086) are engineered for indefinite proliferation while maintaining essential immunological functions. These cells provide a standardized system for studying T cell activation, immune signaling pathways, and adaptive immune response mechanisms. Their consistency and scalability make them a valuable tool for preclinical research in immunotherapy, CAR-T development, and vaccine studies.

Key Features and Benefits

Reliable T Cell Model: Maintains functional characteristics crucial for immune research.
Immune Response Studies: Suitable for analyzing T cell activation, signaling, and interactions.
Scalability: Enables large-scale production for functional and genetic assays.
Preclinical Applications: Extensively used in immunotherapy research, including CAR-T and vaccine development.
Stable & Reproducible: Engineered for consistent experimental results, reducing batch-to-batch variability.

Culture & Handling Guidelines

Recommended Culture Conditions

Culture Vessel: T12.5 culture flasks; avoid using culture vessels with surface areas exceeding 25 cm².
Growth Medium: PriGrow X Series Medium (TM0086) supplemented with 1% Penicillin/Streptomycin (G255).
Incubation: 37°C, 5% CO₂ in a humidified atmosphere.
Activation: Immunocult Human CD3/CD28 T Cell Activator (Stemcell, Cat. 10971) required for cell activation after thawing.

Thawing Protocol

Thaw cells rapidly in a 37°C water bath (maximum 2 minutes). Keep the vial cap above the water level to avoid contamination.
Decontaminate the vial by spraying with 70% ethanol and transfer to a biosafety cabinet.
Add the cell suspension to a 15 mL sterile conical tube containing 5 mL pre-warmed complete medium supplemented with 10% FBS.
Centrifuge at 125xg for 5 minutes and aspirate the supernatant carefully.
Resuspend the cell pellet in 1 mL pre-warmed complete medium and transfer to a T12.5 flask with 3 mL of medium.
Add 25 µL of Immunocult CD3/CD28 T Cell Activator per mL of total volume.
Incubate under recommended conditions and allow cells to grow undisturbed for 3 days. Healthy cells will form clumps.

Subculture Guidelines

Subculture cells when the culture reaches 80-90% confluency.
Transfer cells to T25 flasks if necessary but avoid larger vessels.
Subculture Methods:
- Option 1: Add fresh complete medium directly to the flask.
- Option 2: Transfer half of the cell suspension to a new flask and add fresh medium to both flasks.
Supplement with 20 ng/mL IL-2 for 3 days if cell growth slows. Repeat if needed.
Note: Avoid centrifugation during subculture procedures.

Cryopreservation Guidelines

Cryopreservation Medium: TM024 or 50% complete growth medium, 40% FBS, and 10% DMSO.
Freezing Protocol: Freeze at a controlled rate of -1°C per minute before transferring to liquid nitrogen storage.

Disclaimer

This product is intended for research use only and is not approved for diagnostic, therapeutic, or clinical applications.
Performance data provided are based on standardized protocols and may vary under different experimental conditions.
Sale and use of this product are subject to compliance with any applicable local, national, or international regulations.
Live cell shipments can be requested at an additional cost; feasibility depends on destination and environmental conditions.
The manufacturer is not responsible for improper application or misuse of this product outside of its intended research scope.