Product Details

• Catalog Number: T9313
• Unit Size: 1 × 10⁶ cells / 1.0 ml
• Species: Human (Homo sapiens)
• Tissue: Nerve
• Donor Information: Schwannoma
• Cell Type: Immortalized Schwannoma cells
• Growth Properties: Adherent, epithelial-like
• Biosafety Level: BSL-2
• Storage: Vapor phase of liquid nitrogen or below -130°C
• Shipping: Shipped on dry ice
• Format: Cryopreserved frozen cells

Overview

Immortalized Human Schwannoma Cells (hTERT + SV40) are developed from human Schwannoma cells through the integration of human telomerase reverse transcriptase (hTERT) and Simian Virus 40 (SV40) large T antigen. These cells are an essential model for understanding Schwannoma pathophysiology, Schwann cell biology, and related neural tumorigenesis. Their applications extend to neurobiology and cancer research, providing a robust system for drug development and therapeutic testing.

Key Features and Benefits

• Stable Long-Term Culture: Immortalized for extended culture periods without signs of senescence.
• Reproducible Results: Offers consistent outcomes across experiments.
• Versatile Model: Supports studies in peripheral nerve tumorigenesis and neural drug discovery.
• Optimized Growth Conditions: Adapted to specialized media for maximum cell viability.
• Adherent Growth: Facilitates straightforward culture handling and maintenance.

Culture & Handling Guidelines

Recommended Culture Conditions

• Coating: PriCoat™ T25 Flasks (G299) or Poly-L-Lysine Coating Solution (TM061) for enhanced adhesion.
• Growth Medium: PriGrow III (TM003) with 10% FBS and 1% Penicillin/Streptomycin (G255).
• Incubation Conditions: 37°C in a humidified atmosphere with 5% CO₂.
• Seeding Density: 30,000 – 50,000 cells/cm²
• Doubling Time: 24–36 hours

Thawing Protocol

1. Quickly thaw cells in a 37°C water bath (no more than 2 minutes), ensuring the vial cap stays above water level.
2. Decontaminate the vial with 70% ethanol and transfer it to a biosafety cabinet.
3. Add thawed cells to a 15 ml sterile conical tube with 5 ml pre-warmed growth medium.
4. Centrifuge at 125 × g for 5–7 minutes.
5. Aspirate the supernatant and gently resuspend the cell pellet in fresh complete medium.
6. Plate cells in a T25 culture flask and incubate under recommended conditions.

Subculturing Guidelines

• Subculture cells when 80% confluent using 0.25% Trypsin-EDTA.
• Neutralize with complete growth medium and centrifuge at 125 × g for 5 minutes.
• Resuspend in fresh medium and replate at a 1:2 split ratio.

Cryopreservation Guidelines

• Cryopreservation Medium: Use Cryopreservation Medium (TM024) or complete medium with 10% DMSO.
• Freezing Procedure: Freeze at -1°C per minute before transferring to liquid nitrogen storage.

Short Tandem Repeat (STR) Analysis:

• D5S818: 11, 11
• D13S317: 10, 11
• D7S820: 8, 9
• D16S539: 11, 12
• VWA: 16, 16
• TH01: 9, 9
• AMEL: X, Y
• TPOX: 8, 11
• CSF1PO: 10, 10
• D12S391: 18, 19
• FGA: 18, 25
• D2S1338: 24, 24
• D21S11: 29, 30
• D18S51: 14, 18
• D8S1179: 11, 15
• D3S1358: 17, 18
• D6S1043: 12, 19
• PENTAE: 11, 13
• D19S433: 14, 15
• PENTAD: 9, 9
• D1S1656: 10, 15

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Disclaimer

1. This product is intended for research use only and not for use in human or animal diagnostic or therapeutic procedures.
2. Ensure the product’s suitability for your specific application. Experimental conditions may affect results.
3. This product is shipped under conditions to maintain viability. Proper storage upon receipt is essential.
4. All sales are final. Contact our technical support for any questions about the product’s use or storage.