Product Details

• • Catalog Number: T0843
  • Unit Size: 1×10^6 cells / 1.0 ml
  • Species: Human (Homo sapiens)
  • Tissue: Blood
  • Cell Type: Immortalized Mast Cells
  • Growth Properties: Suspension; may attach in non-upright culture vessels
  • Biosafety Level: BSL-2
  • Storage Conditions: Vapor phase of liquid nitrogen or below -130°C
  • Shipping Conditions: Shipped on dry ice
  • Format: Cryopreserved frozen cells
  • Incubation Conditions: 37°C, 5% CO₂
  • Immortalization Method: Spontaneous

Overview

Immortalized Human Mast Cells (LUVA) are a non-genetically modified mast cell line derived from hematopoietic progenitor cells. They proliferate independently of exogenous growth factors while retaining key mast cell markers, including tryptase, FcεRI, and c-kit. Upon FcεRI cross-linkage, LUVA cells release mediators such as β-hexosaminidase and prostaglandin D2, making them an ideal tool for research into allergic reactions, immune signaling, and drug response studies.

Key Features and Benefits

• Growth Factor Independence: Proliferates without exogenous growth factors.
• Mast Cell Marker Expression: Expresses tryptase, FcεRI, and c-kit.
• FcεRI-Mediated Degranulation: Responds to cross-linkage with β-hexosaminidase and prostaglandin D2 release.
• Stable and Reproducible: Maintains a stable phenotype, ensuring consistency in experimental applications.
• Valuable for Drug Testing: Ideal for evaluating compounds targeting mast cell activation and allergic response.

Culture & Handling Guidelines

Recommended Culture Conditions

• Culture Vessel: Use upright PriCoat™ T25 Flasks (G299) for optimal growth.
• Growth Medium: PriGrow X Series Medium (TM0843) supplemented with: 
  • 2 mM L-glutamine (G275)
  • 1% Penicillin/Streptomycin Solution (G255)
• Incubation: Maintain at 37°C in a humidified atmosphere with 5% CO₂.
• Seeding Density: 100,000 – 300,000 cells/ml.
• Doubling Time: 24-48 hours.

Thawing Protocol

1. Quickly thaw the vial in a 37°C water bath for no more than 2 minutes.
2. Disinfect the vial with 70% ethanol before transferring to a biosafety cabinet.
3. Transfer the thawed cell suspension into a 15 ml sterile conical tube containing 5 ml of pre-warmed complete medium.
4. Centrifuge at 125xg for 5 minutes, discard the supernatant, and gently resuspend the cell pellet in fresh medium.
5. Transfer cells to an upright T25 culture flask and incubate under recommended conditions.

Subculturing Guidelines

• Change the medium every 2-3 days.
• When the culture reaches optimal density, transfer a portion of the cells to fresh medium at a 1:2 to 1:10 split ratio.
• Centrifuge at 125xg for 5 minutes, resuspend in fresh medium, and seed into new culture vessels.

Cryopreservation Guidelines

• Cryopreservation Medium: Use complete growth medium (TM0843) supplemented with 8% DMSO.
• Freezing Procedure: Freeze gradually at -1°C per minute before transferring to liquid nitrogen storage.

Related Products

• Recombinant Human SCF (E. coli) – Z100815
• Recombinant Human TGF Beta-1 (TGFB1) – Z101555
• Recombinant Human TNF – Z101385

Disclaimer

1. This product is for research use only. Not intended for human or animal diagnostic, therapeutic, or clinical applications.
2. The user is responsible for verifying the suitability of this product for their specific experimental conditions.
3. Handling and usage should comply with all relevant biosafety regulations.
4. No warranties, express or implied, are provided regarding the performance of this product outside of the recommended conditions.

References

• Kirshenbaum, Arnold S., et al. “Demonstration that human mast cells arise from a progenitor cell population that is CD34+, c-kit+, and expresses aminopeptidase N (CD13).” Blood, The Journal of the American Society of Hematology 94.7 (1999): 2333-2342.
• Kirshenbaum, Arnold S., et al. “Characterization of novel stem cell factor responsive human mast cell lines LAD 1 and 2 established from a patient with mast cell sarcoma/leukemia; activation following aggregation of FcεRI or FcγRI.” Leukemia research 27.8 (2003): 677-682.
• Laidlaw, Tanya M., et al. “Characterization of a novel human mast cell line that responds to stem cell factor and expresses functional FcεRI.” Journal of allergy and clinical immunology 127.3 (2011): 815-822.