Product Details

• Catalog Number: T0563
• Unit Size: 1×10^6 cells / 1.0 ml
• Species: Human (Homo sapiens)
• Tissue of Origin: Liver
• Cell Type: Immortalized macrophage-like cells
• Growth Properties: Adherent, epithelial morphology
• Immortalization Method: Lentiviral transduction with SV40, SV40T, and hTERT
• Expression Markers: CD68+, CD14+, CD11b-
• Biosafety Level: BSL-2
• Storage: Below -130°C (liquid nitrogen vapor phase)
• Shipping: Shipped on dry ice
• Format: Cryopreserved frozen cells

Overview

Immortalized Human Hepatic Kupffer Cells provide a consistent and well-characterized model for investigating liver-specific immune responses, drug toxicity, and macrophage-mediated inflammation. Engineered for extended proliferation while preserving primary macrophage characteristics, they are valuable for liver disease research and pharmaceutical applications.

Key Features and Benefits

• Stable Hepatic Macrophage Model: Ensures reproducibility in liver-related research.
• Enhanced Proliferation: Extends usability beyond primary Kupffer cells.
• Drug Screening & Toxicology: An ideal tool for hepatotoxicity studies.
• Supports 3D Culture & Co-Culture: Suitable for advanced liver tissue modeling.
• Expression of Key Markers: Maintains macrophage-specific markers (CD68+, CD14+).

Culture & Handling Guidelines

  Recommended Culture Conditions

• Coating: Use PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) for optimal adhesion.
• Growth Medium: PriGrow IV (TM004) supplemented with: 
  • 10% Fetal Bovine Serum (FBS)
  • 1% Penicillin/Streptomycin (G255)
• Incubation Conditions: 37°C, 5% CO₂ in a humidified atmosphere
• Seeding Density: 10,000 – 20,000 cells/cm²
• Doubling Time: Approximately 36 – 48 hours

Thawing Protocol

1. Quickly thaw the vial in a 37°C water bath (maximum 2 minutes). Keep the vial cap above the water level to avoid contamination.
2. Decontaminate the vial exterior with 70% ethanol and transfer it to a biosafety cabinet.
3. Transfer cell suspension to a 15 mL sterile conical tube containing 5 mL of pre-warmed complete growth medium.
4. Centrifuge at 125xg for 5-7 minutes.
5. Aspirate the supernatant without disturbing the pellet. Resuspend the cell pellet in pre-warmed complete growth medium and seed into a T25 culture flask.
6. Incubate under recommended conditions and monitor for recovery.

Subculture Guidelines

1. Aspirate old medium and rinse with PBS.
2. Add 2-3 mL of pre-warmed Gentle Dissociation Solution (TM080) and incubate at 37°C until cells detach (2-10 minutes).
3. Neutralize with an equal volume of complete growth medium.
4. Transfer to a sterile centrifuge tube and centrifuge at 125xg for 5 minutes.
5. Aspirate the supernatant and resuspend the pellet in fresh complete growth medium.
6. Seed cells into new culture vessels at the desired density and incubate under recommended conditions.

Cryopreservation Guidelines

• Cryopreservation Medium: Use Cryopreservation Medium (TM024) or complete growth medium with 10% DMSO.
• Freezing Procedure: Freeze at a controlled rate (-1°C per minute) before transferring to liquid nitrogen storage.

Related Products

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• Recombinant Human TGF Beta-1 (TGFB1) – Z101555

Disclaimer

1. This product is for research use only and is not intended for diagnostic, therapeutic, or human/animal consumption.
2. All cell line data is based on standardized culture conditions and may vary depending on end-user protocols.
3. A Material Transfer Agreement (MTA) must be completed before purchase.
4. We recommend live cell shipments where feasible. For frozen cells, end-users must follow the exact recommended protocol for best viability.
5. No warranties are made regarding the suitability of this product for specific applications beyond those described. All sales are final.