Product Details

• Catalog Number: T0525
• Unit Size: 1 x 10^6 cells / 1.0 mL
• Species: Human (Homo sapiens)
• Tissue of Origin: Peripheral Blood
• Donor Information: Male, 65 years, Caucasian
• Cell Type: Immortalized dendritic cells
• Growth Properties: Suspension
• Immortalization Method: Retroviral and lentiviral-mediated SV40 expression
• Doubling Time: 48-72 hours
• Biosafety Level: BSL-2
• Storage: Below -130°C (liquid nitrogen vapor phase)
• Shipping: Shipped on dry ice
• Format: Cryopreserved frozen cells

Overview

Immortalized Human Dendritic Cells (IHDC) provide a stable and continuous source of dendritic cells for immunological studies. Engineered for sustained proliferation while maintaining key antigen-presenting properties, IHDC is widely used in immune system modeling, therapeutic vaccine development, and tumor immunology research.

Key Features and Benefits

• Long-Term Stability: Immortalized for extended culture duration without senescence.
• Reproducibility: Provides consistent results across multiple experiments.
• Ideal for Immunology Research: Supports studies on antigen presentation, immune cell signaling, and T-cell interactions.
• Optimized Growth Conditions: Adapted to specific media formulations for enhanced viability.
• Suspension Growth Properties: Allows for easy scalability and handling.

Culture & Handling Guidelines

Recommended Culture Conditions

• Culture Vessel: Use PriCoat™ T25 Flasks (G299) for optimal cell culture.
• Growth Medium: PriGrow X Series Medium for T0525 (TM0525) supplemented with:
  • 10% Fetal Bovine Serum (FBS)
  • 1% Penicillin/Streptomycin Solution (G255)
• Incubation Conditions: Maintain at 37°C in a humidified atmosphere with 5% CO₂.
• Seeding Density: 1×10⁵ – 5×10⁵ cells/mL
• Doubling Time: 48-72 hours

Thawing Protocol

1. Quickly thaw frozen cells in a 37°C water bath (maximum 2 minutes), keeping the vial cap above water level.
2. Decontaminate the vial by spraying with 70% ethanol and transfer it to a biosafety cabinet.
3. Transfer the cell suspension into a 15mL sterile conical tube containing 5mL of pre-warmed complete growth medium.
4. Centrifuge at 125xg for 5-7 minutes.
5. Aspirate the supernatant and re-suspend the pellet in pre-warmed complete growth medium.
6. Dispense cells into a T25 culture flask and incubate under recommended conditions.

Subculturing Guidelines

• Add fresh complete medium directly to the culture; do not allow cell density to exceed 1×10⁶ cells/mL.
• Alternatively, replace the medium by centrifugation and re-suspend in fresh medium before seeding new culture vessels.
• Incubate under the recommended conditions.

Cryopreservation Guidelines

• Cryopreservation Medium: Use Cryopreservation Medium (TM024) or complete growth medium with 10% DMSO.

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Disclaimer

1. This product is for research use only and is not intended for therapeutic or diagnostic applications.
2. All test parameters are based on standardized culture conditions. Results may vary depending on laboratory-specific protocols.
3. All sales are final. If cells do not recover under recommended conditions, replacement at cost may be available.
4. Live cell shipment is available upon request but subject to feasibility based on destination climate and logistics.
5. By purchasing this product, the customer agrees to all terms and conditions outlined in the Material Transfer Agreement (MTA), which must be completed before shipment.