Product Details

• Catalog Number: T0539
• Unit Size: 1×10⁶ cells / 1.0 ml
• Species: Human (Homo sapiens)
• Tissue: Heart (Ventricular cells)
• Donor Information: Female, 33 years old, Caucasian
• Cell Type: Immortalized Cardiomyocytes
• Growth Properties: Adherent, multipolar morphology
• Immortalization Method: Lentiviral transduction with CDK4, hTERT, and BMI1
• Expression Markers: ACTN2 and ACTN3
• Biosafety Level: BSL-2
• Storage: -130°C or vapor phase of liquid nitrogen
• Shipping: Shipped on dry ice
• Format: Cryopreserved (Frozen vial)
• Population Doubling Time: 15 – 25 hours
• Seeding Density: 10,000 – 20,000 cells/cm²

Overview

Immortalized Human Cardiomyocytes – CDK4, hTERT, & BMI provide a highly reproducible and scalable in vitro model for cardiovascular research. These cells are genetically modified to enable indefinite proliferation while retaining cardiomyocyte characteristics, making them ideal for functional studies, electrophysiology assays, and cardiotoxicity evaluations.

Key Features and Benefits

• Long-Term Stability: Engineered for indefinite proliferation, reducing variability between passages.
• Authentic Cardiomyocyte Markers: Expresses ACTN2, ACTN3, and puromycin resistance gene.
• Ideal for Drug Discovery & Toxicity Screening: Provides a reliable platform for evaluating cardiovascular drugs and toxicological effects.
• Reproducible & Scalable: Ensures high reproducibility across multiple experiments, optimizing research efficiency.
• Adherent Growth: Simplifies culture maintenance and imaging applications.

Culture & Handling Guidelines

Recommended Culture Conditions

• Coating: PriCoat™ T25 Flasks (G299) or Extracellular Matrix (G422) for adhesion.
• Growth Medium: PriGrow I (TM001) supplemented with: 
  • 10% Fetal Bovine Serum (FBS)
  • 1% Penicillin/Streptomycin Solution (G255)
• Incubation Conditions: 37°C, 5% CO₂.
• Seeding Density: 10,000 – 20,000 cells/cm²
• Doubling Time: 15 – 25 hours

Thawing Protocol

1. Quickly thaw the vial in a 37°C water bath (~2 minutes), agitating gently.
2. Decontaminate the vial with 70% ethanol before handling.
3. Transfer cell suspension into a 15 ml sterile conical tube with 5 ml pre-warmed culture media.
4. Centrifuge at 125 x g for 5-7 minutes, aspirate supernatant, and resuspend in fresh media.
5. Plate cells in a T25 culture flask and incubate under recommended conditions.

Subculturing Guidelines

1. When cells reach ~80% confluency, aspirate media and add 2-3 ml of pre-warmed 0.25% Trypsin-EDTA.
2. Monitor detachment (~2-10 minutes); incubate at 37°C if necessary.
3. Neutralize with an equal volume of complete growth media.
4. Centrifuge at 125 x g for 5 minutes, aspirate supernatant, and resuspend in fresh media.
5. Plate into new culture vessels at an appropriate density and incubate.

Cryopreservation

• Cryopreservation Medium: Use Cryopreservation Medium (TM024) or complete growth medium supplemented with 10% DMSO.
• Freezing Procedure: Freeze at a controlled rate (-1°C per minute) before transferring to liquid nitrogen storage.

Related Products

• Recombinant Human VEGF (121aa) (E. coli) – Z102115
• Recombinant Human Beta-NGF – Z101545
• Recombinant Human TGF Beta-1 (TGFB1) – Z101555
• Recombinant Human IGF1 (E. coli) – Z100385

Disclaimer

1. For Research Use Only. Not intended for human or animal consumption, therapeutic, or diagnostic applications.
2. All test parameters are based on standardized in-house culture conditions and may vary under different laboratory settings.
3. A Material Transfer Agreement (MTA) may be required for purchase.
4. Cell viability is warranted for 30 days upon initiation of culture, following recommended protocols.
5. The provider is not liable for improper usage or application outside intended research purposes.

Reference

1. Kono, Ken, et al. “Development of selective cytotoxic viral vectors for concentration of undifferentiated cells in cardiomyocytes derived from human induced pluripotent stem cells.” Scientific reports 9.1 (2019): 3630.