Human CD56+ Natural Killer (NK) Cells

Product Details

Product Name: Human CD56+ Natural Killer (NK) Cells
Product Type: Cell line
Catalog Number: T4124
Unit Size: 5 × 10⁵ cells / 1.0 ml
Species: Human (Homo sapiens)
Tissue: Peripheral Blood
Donor History: Healthy, normal donor
Cell Type: Primary Natural Killer (NK) Cells
Growth Properties: Suspension, round morphology
Expression Marker: CD56+
Biosafety Level: BSL-2
Storage Conditions: Vapor phase of liquid nitrogen or below -130°C
Shipping Conditions: Shipped on dry ice
Format: Cryopreserved frozen cells

Overview

Human CD56+ Natural Killer (NK) Cells are a crucial component of the innate immune system, playing a key role in immune surveillance and host defense. These primary NK cells are isolated from human peripheral blood and enriched for CD56 expression, ensuring a highly functional population for immunological research, tumor cell targeting, and antiviral studies. They are widely used in cytotoxicity assays, immune response studies, and adoptive cell therapy research.

Key Features and Benefits

Highly Purified Population: CD56+ enriched for consistency in experiments.
Validated Functional Performance: Demonstrates natural cytotoxic activity against target cells.
Ideal for Immunology Research: Supports studies on immune response, tumor interactions, and viral immunity.
Cryopreserved for Long-Term Storage: Maintains viability and functionality upon thawing.
Ready for Culture: Compatible with standard NK cell activation protocols.

Culture & Handling Guidelines

Recommended Culture Conditions

Culture Vessel: Use suspension culture flasks or low-adherence plates to maintain cell viability.
Growth Medium: PriGrow X Series Medium (TM4124) supplemented with:
- 1% Penicillin/Streptomycin Solution (G255)
- 50 ng/mL Recombinant Human IL-4 (Z100545)
- 50 ng/mL Recombinant Human GM-CSF (Z100275)
Incubation Conditions: Maintain at 37°C in a humidified incubator with 5% CO₂.
Seeding Density: 2×10⁴ – 5×10⁴ cells/cm²
Cell Proliferation: Human CD56+ NK Cells do not proliferate in vitro and should be used within the recommended timeframe for optimal viability.

Thawing Protocol

Remove the cryovial from liquid nitrogen storage and immediately transfer it to a 37°C water bath.
Thaw rapidly (within 2 minutes) while gently agitating the vial. Keep the cap above the water level to avoid contamination.
Spray the vial exterior with 70% ethanol, then transfer it into a biosafety cabinet.
Pipette the cell suspension into a 15 mL sterile conical tube containing 5 mL pre-warmed complete growth medium.
Centrifuge at 400 × g for 10 minutes to pellet the cells.
Carefully aspirate the supernatant without disturbing the cell pellet.
Resuspend the cell pellet in fresh pre-warmed complete growth medium and transfer to a suitable culture vessel.
Incubate under recommended conditions for at least 4 hours before use to allow for cell recovery.

Subculturing Guidelines

Human CD56+ NK Cells do not proliferate under standard culture conditions.
It is recommended to use freshly thawed cells without extended passaging.
If expansion is required, consider co-culture with feeder cells or cytokine stimulation protocols.

Cryopreservation Guidelines

Cryopreservation Medium: Not recommended for re-freezing.
Storage Conditions: Cells must be stored in the vapor phase of liquid nitrogen or below -130°C for long-term preservation.
Viability Considerations: Thawed cells should be used immediately for optimal functionality.

Disclaimer

This product is for research use only and is not intended for therapeutic, diagnostic, or human/animal consumption purposes.
The provided growth parameters are based on standardized conditions and may vary depending on user-specific culture conditions.
It is recommended to validate the product’s suitability before use in critical applications.

All sales are final, and no warranty is provided for performance outside of specified conditions.