Overview:

The Advanced MSC Expansion System is an optimized solution designed for the efficient expansion of mesenchymal stem cells (MSCs) derived from various sources, including adipose tissue, bone marrow, and umbilical cord blood. With a well-balanced formulation, the kit ensures rapid cell proliferation while maintaining high viability and low CD142 expression levels. The absence of unwanted jelly-like formations during culture makes it a superior choice for stem cell research and therapeutic development.

Key Features:

• Optimized for diverse MSC sources: Supports adipose-MSC, bone marrow-MSC, and cord blood-MSC.
• High expansion efficiency: Enables faster doubling times for more effective cell proliferation.
• Regulatory-compliant raw materials: Uses US-sourced components that align with industry quality standards.
• Minimized CD142 expression: Enhances safety profiles for translational research.
• Consistent and stable culture conditions: Eliminates unwanted gelatinous formations, ensuring uniform cell growth.
• Long-term storage stability: Maintains integrity under controlled conditions (medium: 2-8°C, hPL: -20°C).

Available Systems:

MSC Expansion System   (Standard)

Catalog Number: GSMSC-100-001

Includes:

• Advanced MSC Culture Medium (with Phenol Red) – 1L (Catalog No: GSBC-MED-001)
• Human Platelet Lysate (hPL) Supplement – 50mL (Catalog No: GSBC-HPL-001)

MSC Expansion System  (phenol Red-Free)

Catalog Number: GSMSC-100-002

Includes:

• Advanced MSC Culture Medium (No Phenol Red) – 1L (Catalog No: GSBC-MED-002)
• Human Platelet Lysate (hPL) Supplement – 50mL (Catalog No: GSBC-HPL-001)

Individual Components:

Advanced MSC Culture Medium

• With Phenol Red (Catalog No: GSBC-MED-001) – 1L
• Without Phenol Red (Catalog No: GSBC-MED-002) – 1L

Key Benefits: Serum-dependent, free from HEPES buffer, optimized for monolayer MSC expansion.

Human Platelet Lysate (hPL) Supplement

Catalog No: GSBC-HPL-001

Size: 50mL

Key Benefits: Xeno-free, requires no additional heparin, ensures batch-to-batch consistency and enhanced MSC growth.

Performance Metrics: (Figure 1 and 2)

• Adipose-derived MSC Doubling Time: ~23.29 hours
• Bone Marrow-derived MSC Doubling Time: ~28.39 hours
• Stable MSC morphology and differentiation potential

Competitive Edge:

Ideal Applications:

• Stem cell therapy research
• Regenerative medicine applications
• Preclinical studies
• Ex vivo MSC expansion protocols

Figure 1: MSC expansion performance

Figure 2: Cell growth morphology

Figure 3: Flow analysis of BM-MSC CD142 expressions