Product Details

• Product Name: Recombinant Human FGF21 (E. coli)
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z101105
• Unit Size: 20 µg
• Species: Human (Homo sapiens)
• Gene Symbol: FGF21
• Gene ID: 26291
• Accession Number: Q9NSA1
• Expression System: E. coli
• Molecular Weight: ~19.7 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from 0.2 μm filtered solution containing Tris and NaCl (pH 8.0)
• Function: Stimulates insulin-independent glucose uptake in adipocytes in presence of β-Klotho; bioactivity validated in NIH/3T3 proliferation assay (ED₅₀ <1 ng/mL)
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human FGF21 is a member of the FGF19 subfamily and acts as a secreted growth factor involved in energy metabolism and tissue homeostasis. Produced in E. coli, this non-glycosylated protein retains potent biological activity, facilitating adipocyte glucose uptake and influencing cell proliferation and differentiation. Its signaling requires the cofactor β-Klotho, and it has been widely studied in metabolic regulation, obesity, diabetes, and tissue regeneration research.

Key Features and Benefits

• Validated Bioactivity: Promotes NIH/3T3 proliferation at low nanogram concentrations (ED₅₀ <1 ng/mL).
• Efficient Expression: Bacterial system ensures consistent and scalable production.
• High Purity & Low Endotoxin: >95% purity and endotoxin <1 EU/µg, suitable for sensitive cell-based applications.
• Metabolic Relevance: Functions in glucose regulation, adipocyte biology, and energy homeostasis.
• Stable Form: Lyophilized powder provides long-term storage flexibility.

Reconstitution Instructions

To ensure optimal performance:

1. Brief Spin: Centrifuge vial to collect all material at the bottom.
2. Reconstitute: Add sterile distilled water to achieve a final concentration ≥0.1 mg/mL.
3. Mix Gently: Swirl until fully dissolved; do not vortex.
4. Application: Dilute into assay buffers or cell culture medium as required.

Quality Control (QC)

• Purity assessed via SDS-PAGE
• Endotoxin level confirmed by LAL assay
• Bioactivity validated in NIH/3T3 proliferation assay

Caution

This product is intended strictly for research use only. It is not to be used in diagnostic or therapeutic applications involving humans or animals.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not intended for diagnostic, therapeutic, or clinical applications.
4. Users are solely responsible for ensuring compliance with regulatory and safety requirements.