Product Details

• Product Name: Recombinant Human Insulin (INS)
• Product Type: Metabolic Hormones & Peptides
• Catalog Number: Z101065
• Unit Size: 250 mg
• Species: Human (Homo sapiens)
• Gene Symbol: INS
• Gene ID: 3630
• Accession Number: P01308
• Source: coli expression system
• Molecular Weight: ~6.0 kDa (mature heterodimer)
• Form: Lyophilized powder
• Purity: ≥ 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White lyophilized powder
• Formulation: Lyophilized from a 0.2 µm-filtered solution without additives
• Function: Demonstrated biological activity in stimulating insulin receptor pathways and glucose uptake in responsive cell lines
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months, with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Insulin is a biologically active hormone central to the regulation of glucose metabolism, lipid synthesis, and protein anabolism. It is composed of two polypeptide chains (A and B) linked by disulfide bonds, mimicking the native human form. In research, it serves as a potent tool for studying insulin receptor (INSR) signaling, PI3K/AKT and MAPK pathway activation, as well as metabolic modulation in cultured cells. This recombinant protein is ideal for applications requiring defined, animal-free supplementation in cell culture and metabolic assays.

Key Features and Benefits

• Biologically Active: Retains native insulin activity, enabling robust stimulation of insulin-responsive pathways.
• Low Endotoxin Level: < 1.0 EU/µg by LAL assay, suitable for sensitive experimental systems.
• High Purity: ≥ 95% verified by SDS-PAGE to ensure consistency and reproducibility.
• Functionally Validated: Demonstrated ability to activate downstream insulin signaling and promote glucose uptake in vitro.
• Stable Lyophilized Form: Supports long-term storage with maintained bioactivity and convenient handling.

Reconstitution Instructions

To ensure optimal protein stability and activity:

1. Centrifuge the Vial: Briefly spin to collect powder at the bottom.
2. Reconstitute: Dissolve in sterile distilled water; for improved solubility, acidify solution to pH 2–3 with dilute acetic acid or HCl to a final concentration of 1–2 mg/mL.
3. Mix Gently: Swirl to dissolve completely; avoid vigorous agitation.
4. Dilute for Use: Further dilute in the desired buffer containing a carrier protein, maintaining ≥ 0.1 mg/mL protein concentration.
5. Storage: Store reconstituted protein at 2–8°C (≤ 1 month) or –20°C (≤ 6 months); avoid freeze–thaw cycles.

Quality Control (QC)

• Purity: Assessed by SDS-PAGE
• Endotoxin: Measured using LAL method
• Bioactivity: Confirmed via receptor activation and metabolic uptake assays in insulin-responsive cells

Caution

This product is intended for research use only. It is not to be used in humans or animals for therapeutic or diagnostic purposes.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not for diagnostic or therapeutic purposes.
4. The supplier assumes no liability for misuse of this product.
5. Users are responsible for compliance with institutional and regulatory guidelines.