Product Details

• Product Name: Recombinant Human IL-33
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z100535
• Unit Size: 10 µg
• Species: Human (Homo sapiens)
• Gene Symbol: IL33
• Gene ID: 90865
• Accession Number: O95760
• Source: E. coli
• Molecular Weight: ~ 18 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White lyophilized powder
• Formulation: Lyophilized from a 0.2 µm-filtered PBS solution
• Function: Demonstrated by dose-dependent proliferation of murine D10S cells; ED₅₀ ~ 0.03 ng/mL
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months, with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Interleukin-33 (IL-33) is a member of the IL-1 cytokine family and functions as a potent inducer of Th2-type immune responses. IL-33 signals through the ST2/IL-1RAcP receptor complex, leading to NF-κB and MAPK (ERK1/2, p38, JNK) activation in mast cells and other immune subsets. It is expressed in a variety of cell types including epithelial cells and fibroblasts, and facilitates enhanced production of IL-5 and IL-13 from Th2 cells. IL-33 also acts as a chemoattractant, contributing to the recruitment of Th2 lymphocytes during allergic and inflammatory responses.

Key Features and Benefits

• Biologically Active: Exhibits high potency with an ED₅₀ of ~0.03 ng/mL based on D10S cell proliferation assay.
• Endotoxin Controlled: < 1.0 EU/µg as per LAL testing—appropriate for sensitive systems.
• High Purity: > 95% verified by SDS-PAGE analysis ensures reproducibility in critical assays.
• Validated Immune Modulation: Activates NF-κB and MAPK signaling; enhances Th2 cytokine responses in vitro.
• Stable Lyophilized Form: Enables long-term storage and consistent performance.

Reconstitution Instructions

To ensure optimal bioactivity and stability:

1. Centrifuge the Vial: Briefly spin down to collect the powder at the bottom.
2. Reconstitute: Add sterile distilled water to a final concentration ≥ 0.1 mg/mL.
3. Mix Gently: Swirl to dissolve; avoid vigorous shaking or vortexing.
4. Dilute for Use: Further dilute in desired buffer containing carrier protein.
5. Storage: Store reconstituted protein at 2–8°C (≤ 1 month) or –20°C (≤ 6 months).

Quality Control (QC)

• Purity: Verified by SDS-PAGE
• Endotoxin: Assessed using Limulus Amebocyte Lysate (LAL) method
• Bioactivity: Confirmed in cell-based proliferation assay with D10S murine cells

Caution

This product is intended for research use only. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not for diagnostic or therapeutic purposes.
4. The supplier assumes no liability for the misuse of this product.
5. Users are responsible for compliance with institutional and regulatory guidelines.