Product Details

• Product Name: Recombinant Human IL-10
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z101495
• Unit Size: 10 µg
• Species: Human (Homo sapiens)
• Gene Symbol: IL10
• Gene ID: 3586
• Accession Number: P22301
• Source: E. coli
• Molecular Weight: ~ 19 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from a 0.2 µm-filtered PBS solution, pH 7.0
• Function: Confirmed by co-stimulation of MC/9 cells in presence of murine IL-4; ED₅₀ ≤ 2.0 ng/mL
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months, with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Interleukin-10 (IL-10) is a dimeric regulatory cytokine that plays an essential role in modulating immune responses. Secreted by various immune cells such as monocytes, activated T lymphocytes, and B cells, IL-10 suppresses inflammatory cytokines like IFN-γ, IL-2, and TNF-β in Th1 cells while preserving Th2 cell functions. This recombinant non-glycosylated IL-10 is produced in E. coli and demonstrates reliable activity in cellular immunoregulatory studies.

Key Features and Benefits

• Functionally Active: Demonstrates potent immunosuppressive activity; ED₅₀ ≤ 2.0 ng/mL in cell-based co-stimulation assays.
• Endotoxin-Safe: LAL-assayed to contain < 1.0 EU/µg; suitable for sensitive biological systems.
• High Purity: Greater than 95% purity verified by SDS-PAGE analysis.
• Proven Bioactivity: Validated in cytokine suppression models with reproducible dose-dependent responses.
• Stable Format: Lyophilized for optimal long-term stability and flexible usage in diverse research settings.

Reconstitution Instructions

To ensure optimal activity and protein integrity:

1. Centrifuge the Vial: Briefly spin to collect the lyophilized powder.
2. Add Sterile Solvent: Reconstitute with sterile distilled water to a final concentration of ≥ 0.1 mg/mL.
3. Mix Gently: Swirl gently to dissolve; do not vortex.
4. Dilute for Application: For experimental use, further dilute in appropriate buffer containing carrier protein.
5. Storage: After reconstitution, store at 2–8°C for short-term use (≤ 1 month), or –20°C for long-term (≤ 6 months).
   • Avoid repeated freeze-thaw cycles.

Quality Control (QC)

• Purity confirmed by SDS-PAGE
• Endotoxin level determined by LAL assay
• Biological activity validated in MC/9 cell assay system

Caution

This product is intended for research purposes only. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not for diagnostic or therapeutic purposes.
4. The supplier assumes no liability for the misuse of this product.
5. Users are responsible for compliance with institutional and regulatory guidelines.