Product Details

• Product Name: Recombinant Human IL-7
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z100975
• Unit Size: 10 µg
• Species: Human (Homo sapiens)
• Gene Symbol: IL7
• Gene ID: 3574
• Accession Number: P13232
• Source: E. coli
• Molecular Weight: ~ 17.7 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from a 0.2 µm-filtered sodium phosphate and NaCl solution
• Function: Validated via dose-dependent proliferation of mouse lymphoma cells; ED₅₀ < 0.5 ng/mL
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months, with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Interleukin-7 (IL-7) is a hematopoietic cytokine critical to lymphocyte development and survival. It is secreted by stromal epithelial cells in the thymus, bone marrow, and intestinal mucosa, and it plays a central role in supporting early T and B cell proliferation. This non-glycosylated IL-7 is produced in E. coli and purified to high homogeneity, offering a reliable reagent for immune and hematopoietic research models.

Key Features and Benefits

• Functionally Active: Potently induces lymphoid proliferation in bioassays (ED₅₀ < 0.5 ng/mL).
• Endotoxin-Safe: LAL-tested, < 1.0 EU/µg, ensuring compatibility with sensitive in vitro applications.
• High Purity: SDS-PAGE verified to exceed 95% purity.
• Proven Performance: Validated in dose-response assays with consistent biological activity.
• Stable Format: Lyophilized preparation supports long-term storage and easy reconstitution.

Reconstitution Instructions

To ensure optimal activity and protein integrity:

1. Centrifuge the Vial: Briefly spin to collect all lyophilized material.
2. Add Sterile Solvent: Reconstitute in sterile distilled water to reach ≥ 0.1 mg/mL final concentration.
3. Mix Gently: Gently swirl to dissolve; do not vortex or shake vigorously.
4. Dilute for Application: For downstream use, dilute in appropriate buffers containing carrier protein.
5. Storage: Store at 2–8°C for short-term use (≤ 1 month), or –20°C for long-term (≤ 6 months).
   • Avoid repeated freeze-thaw cycles.

Quality Control (QC)

• 95% purity confirmed by SDS-PAGE
• Endotoxin levels determined by LAL assay
• Bioactivity confirmed via cell-based functional assays

Caution

This product is intended for research purposes only. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not for diagnostic or therapeutic purposes.
4. The supplier assumes no liability for the misuse of this product.
5. Users are responsible for compliance with institutional and regulatory guidelines.