Product Details

• Product Name: Recombinant Human IL-6
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z100555
• Unit Size: 20 µg
• Species: Human (Homo sapiens)
• Gene Symbol: IL6
• Gene ID: 3569
• Accession Number: P05231
• Source: E. coli
• Molecular Weight: ~ 21 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from a 0.2 µm-filtered PBS solution (pH 7.4)
• Function: Bioactivity validated in TF-1 cell proliferation assay; ED₅₀ = 0.5–1.0 ng/mL
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months, with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Interleukin-6 (IL-6) is a multifunctional cytokine involved in the regulation of immune response, inflammation, acute-phase reactions, hematopoiesis, and oncogenesis. It is produced by numerous cell types including monocytes, T and B lymphocytes, fibroblasts, and endothelial cells upon activation. This recombinant IL-6 protein is expressed in E. coli, non-glycosylated, and functionally active, making it an ideal tool for in vitro modeling of IL-6-dependent signaling pathways.

Key Features and Benefits

• Functionally Active: Stimulates TF-1 cell proliferation in a dose-dependent manner (ED₅₀ = 0.5–1.0 ng/mL).
• Endotoxin-Safe: < 1.0 EU/µg – compatible with sensitive cell-based systems.
• High Purity: > 95% verified by SDS-PAGE.
• Validated Bioactivity: Consistent and reproducible results in IL-6 signaling assays.
• Flexible Storage: Lyophilized form ensures long-term stability and easy reconstitution.

Reconstitution Instructions

To ensure maximum protein activity and stability, follow these steps:

1. Centrifuge the Vial: Briefly spin down to collect lyophilized content.
2. Add Sterile Solvent: Reconstitute with sterile PBS to reach a final concentration ≥ 0.1 mg/mL.
3. Mix Gently: Swirl gently to dissolve. Avoid vigorous shaking or vortexing.
4. Dilute as Needed: Use suitable buffers with carrier protein for further dilution and applications.
5. Storage: Store at 2–8°C (up to 1 month), or –20°C (up to 6 months).
   • Avoid repeated freeze-thaw cycles.

Quality Control (QC)

• Purity confirmed by SDS-PAGE
• Endotoxin levels tested via LAL assay
• Bioactivity verified in TF-1 proliferation assay

Caution

This product is intended for research purposes only. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not for diagnostic or therapeutic purposes.
4. The supplier assumes no liability for misuse of the product.
5. Users are solely responsible for compliance with all applicable regulations.