Product Details

• Product Name: Recombinant Human IL-4
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z100545
• Unit Size: 10 µg
• Species: Human (Homo sapiens)
• Gene Symbol: IL4
• Gene ID: 3565
• Accession Number: P05112
• Source: E. coli
• Molecular Weight: ~ 15 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from a 0.2 µm-filtered solution in sodium phosphate buffer with NaCl (pH 7.8)
• Function: Supports B-cell differentiation and immunoglobulin class switching; regulates Fc receptor expression
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months, with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Interleukin-4 (IL-4) is a key immunomodulatory cytokine produced primarily by activated T helper 2 (Th2) cells. It plays a critical role in B cell proliferation, immunoglobulin class switching (notably to IgE and IgG1), and regulation of monocyte and macrophage responses. This recombinant protein, expressed in E. coli, provides a consistent and high-quality tool for investigating IL-4-mediated signaling pathways and immune responses in vitro.

Key Features and Benefits

• Biological Activity: Promotes B cell activation and enhances class II MHC expression.
• Endotoxin-Safe: < 1.0 EU/µg – suitable for sensitive culture systems.
• High Purity: > 95% verified by SDS-PAGE.
• Functionally Validated: Active in immunomodulatory assays involving Fc receptor regulation.
• Stable & Flexible: Supplied lyophilized for long-term storage and ease of reconstitution.

Reconstitution Instructions

To ensure maximum protein activity and stability, follow these steps:

1. Centrifuge the Vial: Briefly spin down to collect lyophilized content.
2. Add sterile solvent: Reconstitute using distilled water to achieve a final concentration ≥ 0.1 mg/mL.
3. Mix gently: Swirl or tap to dissolve. Avoid vortexing.
4. Dilute as needed: Further dilute in appropriate buffers containing carrier protein for use.
5. Storage: Reconstituted solution can be stored at 2–8°C (up to 1 month), or –20°C (up to 6 months with stabilizer).
   • Avoid repeated freeze-thaw cycles.

Quality Control (QC)

• Purity confirmed by SDS-PAGE
• Endotoxin level tested using LAL assay
• Functionally validated by cytokine activity on immune cell targets

 Caution

This product is intended for research purposes only. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not for diagnostic or therapeutic purposes.
4. The supplier assumes no liability for misuse of the product.
5. Users are solely responsible for compliance with all applicable regulations.