Product Details

• Product Name: Recombinant Human SCF
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z100815
• Unit Size: 10 µg
• Species: Human (Homo sapiens)
• Gene Symbol: KITLG
• Gene ID: 4254
• Accession Number: P21583
• Expression System: E. coli
• Molecular Weight: ~18.7 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White lyophilized powder
• Formulation: Lyophilized from 0.2 µm filtered Tris and NaCl solution
• Function: Potently stimulates proliferation of TF-1 cells; ED₅₀ ≤ 0.1 ng/mL
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Stem Cell Factor (SCF) is a monomeric hematopoietic cytokine that plays a central role in early blood cell development and progenitor cell survival. It binds the KIT receptor (CD117), activating key signaling cascades essential for hematopoiesis. SCF acts synergistically with cytokines like IL-7, GM-CSF, and G-CSF to support lineage expansion and immune cell generation. Produced in E. coli, this recombinant form contains internal disulfide bonds and retains full biological functionality for in vitro studies of stem cell biology, oncology, and regenerative medicine.

Key Features and Benefits

• Validated Activity: Stimulates TF-1 cell proliferation (ED₅₀ ≤ 0.1 ng/mL).
• Defined Structure: Monomeric protein with internal disulfide bonds.
• High Purity and Low Endotoxin: SDS-PAGE validated purity > 95%; endotoxin < 1 EU/µg.
• Broad Research Utility: Suitable for hematopoiesis, immunology, and stem cell expansion studies.
• Stable Lyophilized Format: Ideal for long-term storage and controlled reconstitution.

Reconstitution Instructions

To prepare for use, follow these procedures carefully:

1. Centrifuge the Vial: Briefly spin to ensure powder is collected at the bottom.
2. Reconstitute: Add sterile distilled water to achieve a final concentration of ≥ 0.1 mg/mL.
   • Gently swirl to dissolve completely. Do not vortex.
3. Dilution and Application: Dilute the reconstituted solution into desired buffer or medium as needed.
4. Storage:
   • Store reconstituted solution at 2–8°C for up to 1 month, or –20°C for up to 6 months with carrier protein.
   • Avoid repeated freeze/thaw cycles.

Quality Control (QC)

• Purity confirmed by SDS-PAGE
• Endotoxin level determined by LAL assay
• Bioactivity validated in TF-1 cell proliferation assay

Caution

This product is intended for research purposes only. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not intended for diagnostic or therapeutic applications.
4. No responsibility is assumed for any use other than research.
5. Users are solely responsible for safety and regulatory compliance.