Product Details

• Product Name: Recombinant Human GDNF
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z101055, Z101059
• Unit Size: 10 µg, 1.0 mg
• Species: Human (Homo sapiens)
• Gene Symbol: GDNF
• Accession Number: P39905
• Source: E. coli
• Molecular Weight: ~ 15 kDa (monomer)
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from a 0.2 µm filtered solution containing sodium citrate and NaCl, pH 4.0
• Function: Demonstrates ED₅₀ < 0.1 ng/mL based on proliferation assay in rat C6 glioma cells
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Glial cell line-Derived Neurotrophic Factor (GDNF) is a critical protein for promoting the survival and functional differentiation of dopaminergic neurons. Acting through the RET receptor and GFRα co-receptor system, GDNF plays a vital role in neural development, repair, and disease models. This recombinant protein, produced in E. coli, forms a non-glycosylated, disulfide-linked homodimer and is supplied in a highly purified and biologically validated format for reliable experimental performance.

Key Features and Benefits

• High Activity: Supports dopaminergic neuron survival with ED₅₀ < 0.1 ng/mL.
• Neuroscience-Validated: Suitable for use in models of Parkinson’s disease, neural regeneration, and CNS development.
• High Purity: > 95% purity confirmed by SDS-PAGE.
• Low Endotoxin: Endotoxin level < 1.0 EU/µg for reduced background in sensitive assays.
• Stable and Flexible: Long shelf life when lyophilized and versatile storage options post-reconstitution.
• Recombinant Consistency: Manufactured using recombinant expression for batch-to-batch reproducibility.

Reconstitution Instructions

To prepare for use, follow these procedures carefully:

1. Centrifuge the Vial: Briefly spin to collect the lyophilized content at the bottom.
2. Reconstitute: Add sterile distilled water to achieve a final concentration of not less than 0.1 mg/mL.
   • Gently swirl or tap the vial to fully dissolve the contents. Do not vortex.
3. Dilution and Application: Once reconstituted, the solution can be further diluted into appropriate experimental buffers as needed.
4. Storage: The reconstituted solution is stable at 2–8°C for up to 1 month, or –20°C for up to 6 months with a carrier protein.
   • Avoid repeated freeze/thaw cycles to maintain protein integrity.

Quality Control (QC)

• Purity confirmed by SDS-PAGE
• Endotoxin content verified by LAL assay
• Functional activity confirmed via rat C6 cell proliferation assay

Caution

This product is intended for research purposes only. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not for diagnostic or therapeutic purposes.
4. No liability for improper or unintended use.
5. Users are responsible for appropriate regulatory compliance and safe handling.