Product Details

• Product Name: Recombinant Human EGF
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z100139
• Unit Size: 1.0 mg
• Species: Human (Homo sapiens)
• Gene Symbol: EGF
• Gene ID: 1950
• Accession Number: P01133
• Source: E. coli
• Molecular Weight: ~6.5 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Formulation: Lyophilized from 0.2 µm filtered solution in water-based buffer
• Function: Demonstrates ED₅₀ < 2 ng/mL in HeLa cell proliferation assay
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Epidermal Growth Factor (EGF) is a biologically active polypeptide involved in the regulation of cellular proliferation, differentiation, and survival. EGF exerts its effect through binding to the epidermal growth factor receptor (EGFR), initiating intracellular signaling cascades essential for tissue development, wound repair, and oncogenic processes. Produced in E. coli, this recombinant EGF is functionally validated and ideal for in vitro applications.

Key Features and Benefits

• High Biological Activity: Proven to stimulate HeLa cell proliferation at ED₅₀ < 2 ng/mL
• Consistent Quality: ≥ 95% purity with low endotoxin levels
• Reliable Research Use: Suitable for studies in cell growth, signal transduction, and regenerative medicine
• Batch-to-Batch Consistency: Recombinant production ensures reproducible results
• Stable Formulation: Long-term lyophilized stability with flexibility for post-reconstitution storage

Reconstitution Instructions

To prepare for use, follow these procedures carefully:

1. Centrifuge the Vial:
   • Briefly spin to collect the lyophilized content at the bottom.
2. Reconstitute:
   • Add sterile distilled water to achieve a final concentration of not less than 0.1 mg/mL.
   • Gently swirl or tap to dissolve completely. Do not vortex.
3. Dilution and Application:
   • Once reconstituted, the solution can be diluted into desired experimental buffers.
4. Storage:
   • Reconstituted solution is stable at 2–8°C for 1 month, or –20°C for 6 months.
   • Avoid repeated freeze/thaw cycles.

Quality Control (QC)

• Purity validated by SDS-PAGE
• Endotoxin tested by LAL assay
• Biological activity confirmed through HeLa proliferation assay

Caution

This product is intended for research purposes only. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not for diagnostic or therapeutic purposes.
4. No liability for improper use.
5. Users are responsible for all regulatory compliance and safety precautions.