Product Details

• Product Name: Recombinant Human Beta-NGF
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z101545
• Unit Size: 20 µg
• Species: Human (Homo sapiens)
• Gene Symbol: NGF
• Gene ID: 4803
• Accession Number: P01138
• Expression System: CHO cells
• Molecular Weight: ~27 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from a 0.2 μm filtered solution in 20 mM sodium acetate, 150 mM NaCl, pH 5.5
• Function: Induces TF-1 cell proliferation (ED₅₀ ≈ 0.5 ng/mL)
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Beta-Nerve Growth Factor (Beta-NGF) is a critical neurotrophin involved in the development and maintenance of the peripheral and central nervous systems. This glycoprotein not only supports neuronal differentiation and synaptic plasticity, but also plays important roles in modulating immune responses, cytokine signaling, and gene expression. Synthesized in CHO cells to preserve post-translational modifications, this recombinant form is ideal for research on neurodegeneration, inflammation, and regenerative biology.

Key Features and Benefits

• Validated Bioactivity: Promotes TF-1 cell proliferation at nanogram levels (ED₅₀ ≈ 0.5 ng/mL).
• Mammalian Expression: CHO-derived for authentic protein folding and glycosylation.
• High Purity: >95% as verified by SDS-PAGE.
• Low Endotoxin: <1.0 EU/µg ensures suitability for sensitive assays.
• Multifunctional Role: Relevant in neuroscience, immunology, oncology, and developmental biology.
• Long-Term Stability: Supplied as lyophilized powder for extended shelf life.

Reconstitution Instructions

To prepare for use, follow these procedures carefully:

1. Centrifuge the Vial: Briefly spin to ensure powder is collected at the bottom.
2. Reconstitute: Add sterile distilled water to achieve a final concentration of 0.1 mg/mL or higher.
   • Swirl gently to dissolve completely. Do not vortex.
3. Dilution and Application: Dilute the reconstituted solution with the desired buffer or culture media as needed.
4. Storage: Store reconstituted solution at 2–8°C for up to 1 month, or at –20°C for up to 6 months with a suitable carrier protein. Avoid repeated freeze/thaw cycles.

Quality Control (QC)

• SDS-PAGE confirmed purity ≥ 95%
• Endotoxin tested and ≤ 1.0 EU/µg (LAL method)
• Functional assay validated in TF-1 cell proliferation assay

Caution

This product is intended strictly for research purposes. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for use in humans or veterinary procedures.
3. Not intended for clinical, therapeutic, or diagnostic applications.
4. Users are responsible for compliance with local laws and regulations.