Product Details

• Product Name: Recombinant Human TGFA (121aa)
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z101935
• Unit Size: 20 µg
• Species: Human (Homo sapiens)
• Gene Symbol: TGFA
• Gene ID: 7039
• Accession Number: P01135
• Expression System: E. coli
• Molecular Weight: ~5.8 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE)
• Endotoxin Level: < 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from 0.2 μm filtered solution in Acetonitrile and TFA
• Function: Induces proliferation in Balb/3T3 fibroblasts with ED₅₀ typically between 0.1 – 0.4 ng/mL
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months with carrier protein)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Transforming Growth Factor Alpha (TGFA) is a member of the epidermal growth factor (EGF) family and functions as a potent mitogenic cytokine. TGFA is biosynthesized as a transmembrane precursor and released in its soluble active form via proteolytic cleavage. It is widely expressed during embryonic development and in adult tissues such as brain, skin, and immune cells, and is frequently upregulated in various cancers. This recombinant form is produced in E. coli and maintains robust activity in cell-based proliferation assays.

Key Features and Benefits

• Validated Activity: Stimulates Balb/3T3 cell proliferation at sub-nanogram concentrations (ED₅₀ = 0.1–0.4 ng/mL).
• Efficient Expression System: Cost-effective and scalable production in E. coli.
• High Quality: >95% purity by SDS-PAGE; endotoxin <1 EU/µg.
• Broad Utility: Suitable for studies in cancer biology, developmental signaling, and epithelial tissue research.
• Stable Format: Lyophilized for extended storage and long-term usability.

Reconstitution Instructions

To prepare for use, follow these procedures carefully:

1. Centrifuge the Vial: Briefly spin to ensure powder is collected at the bottom.
2. Reconstitute: Add sterile 10 mM Acetic Acid to achieve a final concentration of 100 µg/mL.
   • Swirl gently to dissolve completely. Do not vortex.
3. Dilution and Application: Dilute the reconstituted solution with the desired buffer or culture media as needed.
4. Storage: Store reconstituted solution at 2–8°C for up to 1 month, or at –20°C for up to 6 months with a suitable carrier protein. Avoid repeated freeze/thaw cycles.

Quality Control (QC)

• Purity confirmed by SDS-PAGE
• Endotoxin level determined by LAL assay
• Bioactivity validated in Balb/3T3 cell proliferation assay

Caution

This product is intended strictly for research purposes. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not intended for diagnostic or therapeutic applications.
4. No responsibility is assumed for any use other than research.
5. Users are solely responsible for safety and regulatory compliance.