Product Details

• Product Name: Recombinant Human HGF
• Product Type: Growth Factors & Cytokines
• Catalog Number: Z102785
• Unit Size: 5 µg
• Species: Human (Homo sapiens)
• Gene Symbol: HGF
• Gene ID: 3082
• Accession Number: P14210
• Expression System: CHO cells
• Molecular Weight: ~79.4 kDa
• Form: Lyophilized powder
• Purity: > 95% (SDS-PAGE & HPLC)
• Endotoxin Level: ≤ 1.0 EU/µg (LAL method)
• Appearance: White to off-white lyophilized powder
• Formulation: Lyophilized from 0.2 µm filtered aqueous solution
• Function: Stimulates proliferation of monkey 4MBr-5 cells, with ED₅₀ typically in the range of 1.0–20.0 ng/mL
• Storage:
  • Lyophilized: –70°C (≥ 12 months)
  • After reconstitution:
    • 2–8°C (≤ 1 month)
    • –20°C (≤ 6 months)
  • Avoid repeated freeze/thaw cycles
• Shipping: Dry ice
• Usage: For research use only; not for diagnostic or therapeutic applications

Overview

Recombinant Human Hepatocyte Growth Factor (HGF) is a multifunctional protein that regulates cell proliferation, motility, angiogenesis, and tissue regeneration through the c-Met receptor. Its activity triggers downstream MAPK and PI3K pathways, contributing to processes ranging from wound healing to cancer metastasis. Produced in a mammalian system （CHO cells), this recombinant HGF maintains post-translational modifications critical to its stability and function in vitro.

Key Features and Benefits

• Validated Activity: Stimulates 4MBr-5 cell proliferation (ED₅₀ = 1–20 ng/mL)
• Physiological Relevance: Mammalian expression supports proper folding and glycosylation
• High Purity and Low Endotoxin: SDS-PAGE/HPLC confirmed purity; endotoxin ≤ 1 EU/µg
• Broad Research Utility: Suitable for cancer biology, regenerative medicine, and morphogenesis studies
• Stable Lyophilized Format: Ideal for long-term storage and flexible reconstitution

Reconstitution Instructions

To prepare for use, follow these procedures carefully:

1. Centrifuge the Vial:
   • Spin briefly to ensure powder is collected at the bottom.
2. Reconstitute:
   • Add sterile distilled water to achieve a final concentration of 0.1–1 mg/mL.
   • Swirl gently to dissolve completely. Do not vortex.
3. Dilution and Application:
   • Dilute reconstituted solution with desired buffer or media as needed.
4. Storage:
   • Store reconstituted solution at 2–8°C for up to 1 month, or –20°C for up to 6 months.
   • Avoid repeated freeze/thaw cycles.

Quality Control (QC)

• Purity confirmed by SDS-PAGE and HPLC
• Endotoxin level determined by LAL assay
• Bioactivity validated in 4MBr-5 cell proliferation assay

Caution

This product is intended for research purposes only. It is not to be used in humans or animals for therapeutic or diagnostic procedures.

Disclaimer

1. For research use only.
2. Not for human or veterinary use.
3. Not intended for diagnostic or therapeutic applications.
4. No responsibility is assumed for any use other than research.
5. Users are solely responsible for safety and regulatory compliance.