Immortalized Human Neuronal Progenitor Cells (Neu41)

Product Details

Catalog Number: T0781
Unit Size: 1×10⁶ cells / 1.0 ml
Species: Human (Homo sapiens)
Tissue Origin: Brain
Donor Information: Not disclosed
Cell Type: Immortalized Neuronal Progenitor Cells
Morphology: Adherent, spindle-shaped
Expression Markers: Beta III tubulin and MAP-2
Biosafety Level: BSL-2
Storage: Vapor phase of liquid nitrogen or below -130°C
Shipping: Shipped on dry ice
Format: Cryopreserved frozen cells

Overview

The Immortalized Human Neuronal Progenitor Cells (Neu41) offer a highly reproducible in vitro model for studying neuronal development, differentiation, and neurobiology. The cells have been immortalized using a non-DNA integrating cytoplasmic content transfer method, preserving key characteristics of primary neuronal progenitor cells. Neu41 can be differentiated into mature neurons using appropriate differentiation conditions, making them an excellent tool for neuroscience research.

Key Features and Benefits

Long-Term Viability: Immortalized for extended culture durations without senescence.
Reproducibility: Consistent performance across multiple experiments.
Ideal for Neurobiology Research: Supports studies on neuronal differentiation, neuroinflammation, and neurodevelopment.
Optimized Growth Conditions: Adapted to specific media and extracellular matrices for enhanced viability.
Adherent Growth Properties: Facilitates easy handling and maintenance in culture.

Culture & Handling Guidelines

Recommended Culture Conditions

Coating: Use PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) for optimal adhesion.
Growth Medium: PriGrow IV (TM004) supplemented with:
- 5% Fetal Bovine Serum (FBS)
- 10 ng/ml Human FGF2 (Z101455)
- 10 ng/ml Human GH1 (Z100265)
- 10 ng/ml Human HGF (Z102775)
- 1% Penicillin/Streptomycin Solution (G255)
Incubation Conditions: Maintain at 37°C in a humidified atmosphere with 5% CO₂.
Seeding Density: 20,000 – 40,000 cells/cm²
Doubling Time: 24 – 30 hours

Thawing Protocol

Quickly thaw cells in a 37°C water bath (maximum 2 minutes). Keep the vial cap above the water level to avoid contamination.
Decontaminate the vial by spraying with 70% ethanol and transfer to a biosafety cabinet.
Add thawed cells to a sterile 15mL conical tube containing 5mL of pre-warmed complete growth medium.
Centrifuge at 125xg for 5-7 minutes.
Aspirate the supernatant, gently resuspend the cell pellet in fresh complete medium, and seed into a pre-coated T25 flask.
Incubate under recommended conditions and allow cells to recover before passaging.

Subculturing Guidelines

Subculture when cells reach 80% confluence.
Detach cells using 0.25% Trypsin-EDTA for 2-10 minutes at 37°C.
Neutralize Trypsin-EDTA with complete growth media.
Centrifuge at 125xg for 5 minutes, resuspend in fresh media, and re-seed as needed.

Cryopreservation Guidelines

Cryopreservation Medium: Use Cryopreservation Medium (TM024) or complete growth medium supplemented with 10% DMSO.
Freezing Procedure: Freeze at a controlled rate (-1°C per minute) before transferring to liquid nitrogen storage.

Disclaimer

This product is intended for research use only and not for diagnostic or therapeutic applications.
The viability and performance of this product are batch-dependent and should be verified under the end-user’s culture conditions.
A Material Transfer Agreement (MTA) may be required for purchase.