Product Details

• Catalog Number: T0429
• Unit Size: 1×10^6 cells / 1.0 ml
• Species: Human (Homo sapiens)
• Tissue of Origin: Liver
• Donor Information: Female, normal liver tissue from liver tumor patients
• Cell Type: Immortalized hepatic stellate cells
• Growth Properties: Adherent
• Immortalization Method: Lentiviral transduction with Lenti-hTERT-Neo Virus and Lenti-SV40 lentivirus
• Expression Markers: PDGFRB, GFAP, Desmin, Nestin, α-SMA, Type I Collagen
• Biosafety Level: BSL-2
• Storage: Below -130°C (liquid nitrogen vapor phase)
• Shipping: Shipped on dry ice
• Format: Cryopreserved frozen cells

Overview

The Immortalized Human Hepatic Stellate Cell Line (LSC-1) is a well-characterized cell model engineered for sustained proliferation while preserving key hepatic stellate cell characteristics. It is widely used in liver disease research, fibrosis modeling, and antifibrotic drug screening. LSC-1 cells demonstrate fibrogenic gene expression and can transition between activated and quiescent states under different culture conditions, making them highly valuable for dynamic liver studies.

Key Features and Benefits

• Fibrosis Research: Facilitates the study of fibrogenic pathways and extracellular matrix remodeling.
• Drug Discovery & Toxicology: Suitable for testing antifibrotic compounds and hepatotoxicity assessments.
• 3D Culture & Co-culture Capabilities: Can revert to a quiescent-like phenotype under specialized conditions.
• Reproducibility: Ensures consistency across experiments with a stable genetic background.
• Optimized Growth Conditions: Adapted to specific media formulations for enhanced viability.

Culture & Handling Guidelines

Recommended Culture Conditions

• Coating: PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) for optimal cell attachment.
• Growth Medium: PriGrow IX Medium (TM019) supplemented with:
  • 10% Fetal Bovine Serum (FBS)
  • 1% Penicillin/Streptomycin Solution (G255)
• Incubation Conditions: 37°C in a humidified atmosphere with 5% CO₂.
• Seeding Density: 10,000 – 15,000 cells/cm².
• Doubling Time: Approximately 48 hours.

Thawing Protocol

1. Quickly thaw the vial in a 37°C water bath while gently agitating (maximum 2 minutes). Ensure the vial cap stays above the water level to prevent contamination.
2. Decontaminate the vial by spraying and wiping with 70% ethanol. Perform all subsequent operations in a biological safety cabinet under aseptic conditions.
3. Transfer the cell suspension to a 15 mL sterile conical tube containing 5 mL of pre-warmed complete growth medium. Centrifuge at 125xg for 5-7 minutes.
4. Aspirate the supernatant and gently re-suspend the cell pellet in fresh pre-warmed complete growth medium.
5. Dispense the cell suspension into a T25 culture flask and incubate at the recommended conditions.

Subculture Protocol

1. Aspirate the old medium and rinse cells with PBS.
2. Add 2-3 mL of pre-warmed 0.05% Trypsin-EDTA and incubate at 37°C for 2-10 minutes until cells detach. Observe under a microscope to confirm detachment.
3. Neutralize Trypsin-EDTA with an equal volume of complete growth medium.
4. Transfer the cell suspension to a sterile centrifuge tube and centrifuge at 125xg for 5 minutes.
5. Aspirate the supernatant and re-suspend the cell pellet in fresh complete growth medium.
6. Seed cells at the desired density into new culture vessels and incubate under the recommended conditions.

Cryopreservation

• Cryopreservation Medium: TM024 or complete growth medium with 10% DMSO.
• Freezing Protocol: Freeze at a controlled rate of -1°C per minute before transferring to liquid nitrogen storage.

Related Products

• Recombinant Human TGF-β1 – Z101555
• Recombinant Human Connective Tissue Growth Factor (CTGF) – Z102025)
• Recombinant Human FGF2 – Z101455)

Disclaimer

• This product is intended for research use only and is not approved for diagnostic, therapeutic, or clinical applications.
• Users are responsible for determining the suitability of this product for their specific application.
• Cells must be handled under Biosafety Level 2 (BSL-2) containment following institutional guidelines.
• No warranties are provided regarding performance beyond the described specifications.

References

• Lee, Ho‐Joon, et al. “In vitro modeling of liver fibrosis with 3D co‐culture system using a novel human hepatic stellate cell line.” Biotechnology and Bioengineering 120.5 (2023): 1241-1253.