Immortalized Human Adult Brain Astrocytes (AST-1)

Product Details

Catalog Number: T0783
Unit Size: 1 x 10⁶ cells / 1.0 mL
Species: Human (Homo sapiens)
Tissue: Brain
Donor Information: Not disclosed
Cell Type: Immortalized astrocytes
Growth Properties: Adherent, spindle-shaped
Biosafety Level: BSL-2
Storage: Vapor phase of liquid nitrogen or below -130°C
Shipping: Shipped on dry ice
Format: Cryopreserved frozen cells

Overview

Immortalized Human Adult Brain Astrocytes (AST-1) provide a robust platform for investigating astrocyte functions in neurobiology. These cells were immortalized using hTERT and SV40 genes, allowing extended culture periods and enhanced research reproducibility. AST-1 cells are ideal for studies in neurodegeneration, brain injury response, and glial cell signaling, making them an essential tool for neuroscience research.

Key Features and Benefits

Long-Term Viability: Immortalized for extended culture duration without senescence.
Reproducibility: Provides consistent results across multiple experiments.
Ideal for Neurobiology Research: Supports studies on astrocyte-neuron interactions, neuroinflammation, and neurodegeneration.
Optimized Growth Conditions: Adapted to specific media and extracellular matrices for enhanced viability.
Adherent Growth Properties: Facilitates easy handling and maintenance in culture.

Culture & Handling Guidelines

Recommended Culture Conditions

Coating: Use PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) for optimal adhesion.
Growth Medium: PriGrow IV (TM004) supplemented with:
- 10% Fetal Bovine Serum (FBS)
- 10 ng/mL Human EGF (Z100139)
- 10 ng/mL Human HGF (Z102775)
- 10 ng/mL Human IGF-1 (Z100385)
- 1% Penicillin/Streptomycin (G255)
Incubation Conditions: Maintain at 37°C in a humidified atmosphere with 5% CO₂.
Seeding Density: 20,000 – 40,000 cells/cm²
Doubling Time: ~5 days

Thawing Protocol

Quickly thaw cells in a 37°C water bath (maximum 2 minutes). Keep the vial cap above water level to avoid contamination.
Decontaminate the vial by spraying with 70% ethanol and transfer to a biosafety cabinet.
Add thawed cells to a sterile 15mL conical tube containing 5mL of pre-warmed complete growth medium.
Centrifuge at 125xg for 5-7 minutes.
Aspirate the supernatant, gently resuspend the cell pellet in fresh complete medium, and seed into a pre-coated T25 flask.
Incubate under recommended conditions and allow cells to recover before passaging.

Subculturing Guidelines

Aspirate the old medium and rinse cells with PBS.
Add 2-3 mL of pre-warmed 0.25% Trypsin-EDTA and incubate at 37°C until cells detach (~2-10 minutes).
Neutralize trypsin by adding an equal volume of complete medium.
Centrifuge at 125xg for 5 minutes, aspirate the supernatant, and resuspend the pellet in fresh growth medium.
Seed cells at the appropriate density into new culture vessels.
Incubate under recommended conditions and monitor for confluency.

Cryopreservation Guidelines

Cryopreservation Medium: Use Cryopreservation Medium (TM024) or complete growth medium supplemented with 10% DMSO.
Freezing Protocol: Freeze at a controlled rate (-1°C per minute) before transferring to liquid nitrogen storage.

STR Profiling (Short Tandem Repeat Analysis)

D5S818: 11,13
D13S317: 8,8
D7S820: 8,10
D16S539: 9,13
VWA: 17,17
TH01: 6,6
AMEL: X
TPOX: 8,12
CSF1PO: 10,10
D21S11: 30,31.2

Disclaimer

This product is intended for research use only and is not for therapeutic, diagnostic, or clinical use in humans or animals.
The buyer assumes all responsibility for ensuring that this product meets the requirements of their intended applications and culture conditions.
All test parameters are derived from standard in-house procedures; variations in experimental conditions may yield different results.
Any material resulting from the use of this product should be properly cited in scientific publications.
This product is subject to a Material Transfer Agreement (MTA). Contact technical support for licensing inquiries.