Product Details

• Catalog Number: T0417
• Unit Size: 1 x 10⁶ cells / 1.0 mL
• Species: Human (Homo sapiens)
• Tissue Origin: Peripheral Blood (Immune)
• Donor Information: Male, 28 years old, Caucasian
• Cell Type: Immortalized CD4+ Helper T Cells
• Growth Properties: Suspension
• Expression marker: CD4+
• Biosafety Level: BSL-2
• Storage: Vapor phase of liquid nitrogen or below -130°C
• Shipping: Shipped on dry ice
• Format: Cryopreserved frozen cells

Overview

Immortalized Human Peripheral Blood CD4+ Helper T Cell Line – SV40 serves as a robust model for exploring immune responses. These cells were immortalized using Simian Virus 40 (SV40) large T antigen, ensuring long-term viability and reproducibility. This cell line is suitable for various research applications such as T cell activation, cytokine production, and immune regulation.

Key Features and Benefits

• Long-Term Viability: Immortalized with SV40 for extended culture without senescence.
• Reproducible Results: Consistent and reliable for multiple experimental setups.
• Ideal for Immune Research: Supports studies on T cell activation, cytokine production, and immune cell interactions.
• Optimized Growth Conditions: Adapted for suspension culture in specific media.
• Convenient Handling: Suspension growth properties enable straightforward culture maintenance.

Culture & Handling Guidelines

Recommended Culture Conditions

• Culture Vessels: Use PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) for optimal cell culture.
• Growth Medium: PriGrow X Series Medium for T0417 (TM0417) supplemented with:
  • 25 μl Immunocult Human CD3/CD28 T Cell Activator (Stemcell, Cat.10971) per 10⁶ T cells (only required during initial recovery phase).
• Incubation Conditions: Maintain at 37°C in a humidified atmosphere with 5% CO₂.
• Seeding Density: 2.0 – 5.0 x 10⁵ cells/mL
• Doubling Time: ~24-48 hours

Thawing Protocol

1. Quickly thaw the vial in a 37°C water bath while gently agitating (maximum 2 minutes). Ensure the vial cap remains above water level to prevent contamination.
2. Decontaminate the vial by spraying and wiping its exterior with 70% ethanol. All subsequent steps should be performed inside a biological safety cabinet under aseptic conditions.
3. Transfer the thawed cell suspension into a sterile 15 mL conical tube containing 5 mL of pre-warmed complete growth medium.
4. Centrifuge at 125xg for 5-7 minutes.
5. Aspirate the supernatant carefully without disturbing the cell pellet. Re-suspend the pellet in fresh pre-warmed complete growth medium and transfer to a T25 culture flask.
6. Incubate under recommended conditions and monitor cell viability.
7. Transfer the cells to a pre-coated T25 flask and incubate under recommended conditions.

Subculturing Guidelines

1. Transfer the cell suspension to a sterile conical tube and centrifuge at 125xg for 5 minutes.
2. Aspirate the supernatant carefully and re-suspend the pellet in fresh complete growth medium.
3. Seed the cells into new culture vessels at an appropriate density.
4. Incubate under recommended conditions and monitor cell growth.

Cryopreservation Guidelines

• Cryopreservation Medium: Use Cryopreservation Medium (TM024) or complete growth medium supplemented with 10% DMSO.
• Freezing Protocol:
  1. Prepare a cell suspension in cryopreservation medium at the recommended density.
  2. Aliquot into cryovials and freeze gradually at -1°C per minute before transferring to liquid nitrogen storage.
• Storage: Maintain in the vapor phase of liquid nitrogen or at temperatures below -130°C. Avoid storage at -70°C as it will result in loss of viability.

Related Products

• Recombinant Human IL-2 (E. coli) – Z100485
• Recombinant Human IL-4 (E. coli) – Z100545
• Recombinant Human TGF Beta-1 (E. coli) – Z101555
• Recombinant Human IFN-Gamma (E. coli) – Z101175

Disclaimer

1. This product is intended for research use only and is not approved for therapeutic, diagnostic, or clinical use in humans or animals.
2. The buyer is responsible for ensuring that the product meets the requirements of their specific applications and culture conditions.
3. All test parameters were derived from in-house procedures; results may vary under different conditions.
4. Any material resulting from the use of this product should be properly cited in scientific publications.
5. This product is subject to a Material Transfer Agreement (MTA). Contact technical support for licensing inquiries