Overview:
The NK Cell Expansion System is a cutting-edge solution designed to facilitate the efficient proliferation of highly pure and active Natural Killer (NK) cells from peripheral blood mononuclear cells (PBMCs) or other sources. This system streamlines the expansion process by eliminating the need for feeder cells, antibody coatings, or prior NK cell purification.
Key Features and Benefits:
- Exceptional Expansion Efficiency: Achieves over 3000-fold increase in NK cell numbers within a 14-day culture period.
- High Purity Output: Produces NK cell populations with purity exceeding 90% (CD3⁻CD56⁺).
- Feeder-Free Protocol: Simplifies the expansion process by removing the requirement for feeder cell layers.
- User-Friendly Procedure: Allows direct culturing of PBMCs without the necessity for initial NK cell isolation or antibody coating steps.
- Demonstrated Cytotoxicity: Expanded NK cells exhibit potent cytotoxic activity, with over 80% effectiveness against various cancer cell lines.
Performance Data:
Expansion Metrics
- NK Cell Expansion: Over 3000-fold increase.
- Total Cell Expansion: Exceeds 300-fold.
- Purity Levels: Greater than 90% CD3⁻CD56⁺ NK cells. (Figure 1)
Cytotoxic Activity
- Against K562 cells (E:T=10): 20-70% cytotoxicity.
- Against multiple solid tumor cell lines (E:T=10): Over 80% cytotoxicity.
- Tested on cell lines including MCF-7 (Breast), H441 (Lung), PA-1 (Ovarian), HCT116 (Colon), and MDA-MB-231 (Breast). (Figure 2)
Streamlined Workflow:
- Day 0: Collect and prepare PBMCs (no prior purification required).
- Culture: Initiate culture using the provided Expansion Medium.
- Day 14: Harvest a highly pure NK cell population with substantial expansion.
Competitive Advantages:
| Feature | NK Cell Expansion System | Other Market Solutions |
| Feeder Cells Requirement | Not Required | Often Required |
| Antibody Coating Necessity | Not Needed | Commonly Needed |
| Pre-Expansion NK Cell Purification | Unnecessary | Typically Necessary |
| Expansion Fold Increase | >3000-fold | Generally 100-600-fold |
| NK Cell Purity | >90% | Approximately 40-80% |
| Additional Reagents | None Required | Usually Required |
Kit Components:
- Expansion Medium: 1 Liter
- Supplement: 2 Vial
- Activation Factors: Pre-mixed for convenience
Storage & Stability:
- Storage Conditions: Maintain at 2-8°C.
- Shelf Life: Stable for up to 6 months under recommended conditions.
Handling: Avoid multiple freeze-thaw cycles to preserve component integrity.
Figure 1: NK Cell expansion performance
Figure 2: Cancer cell cytotoxic activity
